4.7 Article

Efficient CRISPR-Cas9-mediated mutagenesis in primary human B cells for identifying plasma cell regulators

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Review Dermatology

B- and Plasma Cell Subsets in Autoimmune Diseases: Translational Perspectives

Van Duc Dang et al.

Summary: B lymphocytes have a critical role in immunity, and in autoimmune diseases, they can exhibit both pathogenic and protective functions. Understanding the different subsets of B cells with these functions can facilitate the development of more specific and personalized therapies.

JOURNAL OF INVESTIGATIVE DERMATOLOGY (2022)

Article Immunology

CD39 and CD326 Are Bona Fide Markers of Murine and Human Plasma Cells and Identify a Bone Marrow Specific Plasma Cell Subpopulation in Lupus

Van Duc Dang et al.

Summary: A large-scale protein screening identified twelve new molecules specifically expressed by murine ASCs, with stable expression of CD39, CD81, CD130, and CD326 offering improved resolution for ASC identification. Further analysis revealed a subpopulation of ASC in autoimmunity expressing high levels of CD39 and CD326.

FRONTIERS IN IMMUNOLOGY (2022)

Article Immunology

Human IgA-Expressing Bone Marrow Plasma Cells Characteristically Upregulate Programmed Cell Death Protein-1 Upon B Cell Receptor Stimulation

Annika Wiedemann et al.

Summary: The study revealed distinct characteristics of IgA(+) BMPC in response to BCR stimulation, showing higher sensitivity to changes in the checkpoint molecule PD-1 compared to other PC subsets regardless of CD19 expression. This indicates that IgA(+) BMPC may have different regulatory functions independent of their phenotypic heterogeneity based on CD19 expression.

FRONTIERS IN IMMUNOLOGY (2021)

Article Biochemical Research Methods

Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

Rebecca Caeser et al.

Summary: This protocol outlines detailed methods for purifying and expanding nonmalignant human germinal center B cells, as well as high-efficiency transduction and CRISPR-Cas9-mediated approaches for genetic manipulation. Mimicking genetic changes commonly found in lymphoid malignancies can lead to the generation of genetically customized, synthetic models of human lymphoma.

NATURE PROTOCOLS (2021)

Article Microbiology

Highly efficient CRISPR-Cas9-mediated gene knockout in primary human B cells for functional genetic studies of Epstein-Barr virus infection

Ezgi Akidil et al.

Summary: Efficient genetic manipulation in resting human B cells has been achieved using Cas9 ribonucleoprotein complexes, followed by EBV infection or culture on CD40 ligand feeder cells. This approach enables specific gene targeting in quiescent human B cells, supporting basic research and immunotherapeutic strategies.

PLOS PATHOGENS (2021)

Review Biochemistry & Molecular Biology

Recent Advances in CRISPR/Cas9 Delivery Strategies

Bon Ham Yip

BIOMOLECULES (2020)

Article Multidisciplinary Sciences

Genetic modification of primary human B cells to model high-grade lymphoma

Rebecca Caeser et al.

NATURE COMMUNICATIONS (2019)

Review Oncology

Targeting transcription factors in cancer - from undruggable to reality

John H. Bushweller

NATURE REVIEWS CANCER (2019)

Article Biochemical Research Methods

Genetic engineering in primary human B cells with CRISPR-Cas9 ribonucleoproteins

Chung-An M. Wu et al.

JOURNAL OF IMMUNOLOGICAL METHODS (2018)

Article Multidisciplinary Sciences

Engineering of Primary Human B cells with CRISPR/Cas9 Targeted Nuclease

Matthew J. Johnson et al.

SCIENTIFIC REPORTS (2018)

Article Biochemical Research Methods

Impact of Signal Peptides on Furin-2A Mediated Monoclonal Antibody Secretion in CHO Cells

Jian'er Lin et al.

BIOTECHNOLOGY JOURNAL (2017)

Article Multidisciplinary Sciences

Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector

Ziqing Liu et al.

SCIENTIFIC REPORTS (2017)

Article Biotechnology & Applied Microbiology

Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes

Van Trung Chu et al.

BMC BIOTECHNOLOGY (2016)

Article Multidisciplinary Sciences

Efficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line

Van Trung Chu et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2016)

Article Biotechnology & Applied Microbiology

Inducible in vivo genome editing with CRISPR-Cas9

Lukas E. Dow et al.

NATURE BIOTECHNOLOGY (2015)

Review Immunology

The generation of antibody-secreting plasma cells

Stephen L. Nutt et al.

NATURE REVIEWS IMMUNOLOGY (2015)

Article Biochemistry & Molecular Biology

CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling

Randall J. Platt et al.

Article Biotechnology & Applied Microbiology

Efficient Lentiviral Transduction and Transgene Expression in Primary Human B Cells

Ulrike Mock et al.

HUMAN GENE THERAPY METHODS (2012)

Article Biotechnology & Applied Microbiology

An antibody delivery system for regulated expression of therapeutic levels of monoclonal antibodies in vivo

Jianmin Fang et al.

MOLECULAR THERAPY (2007)

Article Biotechnology & Applied Microbiology

Stable antibody expression at therapeutic levels using the 2A peptide

JM Fang et al.

NATURE BIOTECHNOLOGY (2005)

Article Multidisciplinary Sciences

Plasma cell differentiation requires the transcription factor XBP-1

AM Reimold et al.

NATURE (2001)