4.1 Article

Evaluating species richness using proteomic fingerprinting and DNA barcoding-a case study on meiobenthic copepods from the Clarion Clipperton Fracture Zone

期刊

MARINE BIODIVERSITY
卷 52, 期 6, 页码 -

出版社

SPRINGER HEIDELBERG
DOI: 10.1007/s12526-022-01307-y

关键词

Meiofauna; Rapid biodiversity assessment; Deep-sea mining; Monitoring; Proteome fingerprint; MALDI-TOF; Barcoding; COI; Copepoda

资金

  1. Projekt DEAL
  2. German Federal Ministry of Education and Research (BMBF)
  3. DFG initiative 1991 Taxon-omics [03F0812E, 03F0707E]
  4. [RE2808/3-1]
  5. [RE2808/3-2]

向作者/读者索取更多资源

This study successfully applied DNA barcoding and MALDI-TOF MS methods to investigate the diversity of meiobenthic copepods in the eastern CCZ. Comparisons of different species delimitation tools showed similar biodiversity values, but differences in species clusters were found, likely due to the high number of rare species represented by only one specimen.
The Clarion Clipperton Fracture Zone (CCZ) is a vast deep-sea region harboring a highly diverse benthic fauna, which will be affected by potential future deep-sea mining of metal-rich polymetallic nodules. Despite the need for conservation plans and monitoring strategies in this context, the majority of taxonomic groups remain scientifically undescribed. However, molecular rapid assessment methods such as DNA barcoding and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) provide the potential to accelerate specimen identification and biodiversity assessment significantly in the deep-sea areas. In this study, we successfully applied both methods to investigate the diversity of meiobenthic copepods in the eastern CCZ, including the first application of MALDI-TOF MS for the identification of these deep-sea organisms. Comparing several different species delimitation tools for both datasets, we found that biodiversity values were very similar, with Pielou's evenness varying between 0.97 and 0.99 in all datasets. Still, direct comparisons of species clusters revealed differences between all techniques and methods, which are likely caused by the high number of rare species being represented by only one specimen, despite our extensive dataset of more than 2000 specimens. Hence, we regard our study as a first approach toward setting up a reference library for mass spectrometry data of the CCZ in combination with DNA barcodes. We conclude that proteome fingerprinting, as well as the more established DNA barcoding, can be seen as a valuable tool for rapid biodiversity assessments in the future, even when no reference information is available.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.1
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据