Genome-Wide Analysis of Specific PfR2R3-MYB Genes Related to Paulownia Witches' Broom

Paulownia witches' broom (PaWB), caused by phytoplasmas, is the most devastating infectious disease of Paulownia. R2R3-MYB transcription factors (TF) have been reported to be involved in the plant's response to infections caused by these pathogens, but a comprehensive study of the R2R3-MYB genes in Paulownia has not been reported. In this study, we identified 138 R2R3-MYB genes distributed on 20 chromosomes of Paulownia fortunei. These genes were classified into 27 subfamilies based on their gene structures and phylogenetic relationships, which indicated that they have various evolutionary relationships and have undergone rich segmental replication events. We determined the expression patterns of the 138 R2R3-MYB genes of P. fortunei by analyzing the RNA sequencing data and found that PfR2R3-MYB15 was significantly up-regulated in P. fortunei in response to phytoplasma infections. PfR2R3-MYB15 was cloned and overexpressed in Populus trichocarpa. The results show that its overexpression induced branching symptoms. Subsequently, the subcellular localization results showed that PfR2R3-MYB15 was located in the nucleus. Yeast two-hybrid and bimolecular fluorescence complementation experiments showed that PfR2R3-MYB15 interacted with PfTAB2. The analysis of the PfR2R3-MYB15 gene showed that it not only played an important role in plant branching, but also might participate in the biosynthesis of photosystem elements. Our results will provide a foundation for future studies of the R2R3-MYB TF family in Paulownia and other plants.

Automated summary

This study identified 138 R2R3-MYB genes in Paulownia, which were classified into 27 subfamilies based on gene structures and phylogenetic relationships. The upregulation of PfR2R3-MYB15 in response to phytoplasma infections was observed, and its overexpression induced branching symptoms in another plant species. PfR2R3-MYB15 was found to interact with PfTAB2 and may participate in biosynthesis of photosystem elements. These findings lay a foundation for future investigations on the R2R3-MYB TF family in Paulownia and other plants.