Quantitative proteomic analysis reveals the effects of mu opioid agonists on HT22 cells

Introduction: At present, the mu opioid receptor is the most important neuroaesthetics receptor in anesthesiology research, and the damage that it does to the nervous system is unknown. Methods: We investigated the effects of loperamide, an agonist of the mu opioid receptor, on protein expression in HT22 cells using stable isotope labeling of amino acids in cell culture (SILAC), immobilized metal affinity chromatography (IMAC) enrichment, and high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 7,823 proteins were identified. Results and Discussion: Bioinformatic analysis revealed that mu opioid receptor agonism can induce distinct changes in the proteome of HT22 cells. These findings improve our understanding of narcotic drugs.

Automated summary

Mu opioid receptor is currently the most important neuroaesthetics receptor in anesthesiology research, and its damage to the nervous system is still unknown. In this study, the effects of loperamide, an agonist of the mu opioid receptor, on protein expression in HT22 cells were investigated using SILAC, IMAC enrichment, and LC-MS/MS. A total of 7,823 proteins were identified. Bioinformatic analysis revealed distinct changes in the proteome of HT22 cells induced by mu opioid receptor agonism. These findings contribute to our understanding of narcotic drugs.