4.5 Article

PP2A phosphatase regulates cell-type specific cytoskeletal organization to drive dendrite diversity

期刊

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fnmol.2022.926567

关键词

dendrite; cytoskeleton; protein phosphatase 2A; Drosophila; microtubules; transcriptional regulation; neuronal polarity

资金

  1. NIH
  2. GSU Molecular Basis of Diseases Fellowship [R01 NS086082, R01 NS039600]
  3. GSU 2CI Fellowship
  4. Kenneth W. and Georganne F. Honeycutt Fellowship
  5. GSU Brains and Behavior Fellowship

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This research revealed the role of PP2A phosphatase in regulating dendrite development in Drosophila neurons. The PP2A complex has cell-type specific regulatory effects and is involved in regulating cytoskeletal organization and organelle localization.
Uncovering molecular mechanisms regulating dendritic diversification is essential to understanding the formation and modulation of functional neural circuitry. Transcription factors play critical roles in promoting dendritic diversity and here, we identify PP2A phosphatase function as a downstream effector of Cut-mediated transcriptional regulation of dendrite development. Mutant analyses of the PP2A catalytic subunit (mts) or the scaffolding subunit (PP2A-29B) reveal cell-type specific regulatory effects with the PP2A complex required to promote dendritic growth and branching in Drosophila Class IV (CIV) multidendritic (md) neurons, whereas in Class I (CI) md neurons, PP2A functions in restricting dendritic arborization. Cytoskeletal analyses reveal requirements for Mts in regulating microtubule stability/polarity and F-actin organization/dynamics. In CIV neurons, mts knockdown leads to reductions in dendritic localization of organelles including mitochondria and satellite Golgi outposts, while CI neurons show increased Golgi outpost trafficking along the dendritic arbor. Further, mts mutant neurons exhibit defects in neuronal polarity/compartmentalization. Finally, genetic interaction analyses suggest beta-tubulin subunit 85D is a common PP2A target in CI and CIV neurons, while FoxO is a putative target in CI neurons.

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