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Zebrafish and medaka T1R (taste receptor type 1) proteins mediate highly sensitive recognition of l-proline

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FEBS OPEN BIO
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WILEY
DOI: 10.1002/2211-5463.13558

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amino acid; l-proline; medaka fish; taste receptor; zebrafish

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In vertebrates, nutritional tastants are recognized by taste receptor type 1 (T1R) family. Zebrafish and medaka fish have oral receptors highly sensitive to amino acids, especially l-proline. Functional assays confirmed the presence of specific T1Rs in these fish, reflecting the physiological importance of l-proline in teleosts.
In vertebrates, nutritional tastants, such as amino acids and sugars, are recognized by G-protein-coupled receptors of the taste receptor type 1 (T1R) family. Previous studies have shown that fish T1Rs are functionally distinct from mammalian T1Rs in certain regards. Here, we report the existence of oral receptors with high sensitivity to amino acids in zebrafish and medaka fish. We describe the construction of multiple cell lines stably expressing functional T1Rs (from medaka fish or zebrafish) with a chimeric G-protein (G16gust44) using the Flp-In system. Through functional assays with these cell lines, medaka fish and zebrafish were confirmed to possess particular T1Rs highly sensitive to l-proline, possibly reflecting the physiological importance of l-proline in teleosts, in line with previous studies.

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