期刊
EMERGING MICROBES & INFECTIONS
卷 12, 期 1, 页码 -出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/22221751.2022.2149353
关键词
Nanoparticle vaccine; SARS-CoV-2; COVID-19; durable antibody response; cross-protectivity; variants of concern
This study developed a subunit vaccine, ASD254, encapsulating the SARS-CoV-2 spike receptor-binding domain (RBD) protein using a nanoparticle vaccine platform. ASD254 showed higher RBD-specific antibody titers and generated significantly more neutralizing antibodies compared to the aluminum-adjuvant RBD vaccine. Mice vaccinated with ASD254 exhibited protective immune responses against SARS-CoV-2, with no detectable viral loads and reduced lung lesions, and the neutralizing antibodies lasted for at least one year and were effective against various SARS-CoV-2 variants.
Numerous vaccines have been developed to address the current COVID-19 pandemic, but safety, cross-neutralizing efficacy, and long-term protectivity of currently approved vaccines are still important issues. In this study, we developed a subunit vaccine, ASD254, by using a nanoparticle vaccine platform to encapsulate the SARS-CoV-2 spike receptor-binding domain (RBD) protein. As compared with the aluminum-adjuvant RBD vaccine, ASD254 induced higher titers of RBD-specific antibodies and generated 10- to 30-fold more neutralizing antibodies. Mice vaccinated with ASD254 showed protective immune responses against SARS-CoV-2 challenge, with undetectable infectious viral loads and reduced typical lesions in lung. Besides, neutralizing antibodies in vaccinated mice lasted for at least one year and were effective against various SARS-CoV-2 variants of concern, including B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), B.1.617.2 (Delta), and B.1.1.529 (Omicron). Furthermore, particle size, polydispersity index, and zeta-potential of ASD254 remained stable after 8-month storage at 4 degrees C. Thus, ASD254 is a promising nanoparticle vaccine with good immunogenicity and stability to be developed as an effective vaccine option in controlling upcoming waves of COVID-19.
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