期刊
BIOLOGY OPEN
卷 11, 期 12, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/bio.059666
关键词
Amnesia; General anesthesia; Sevoflurane; Hippocampus; Neuronal ensemble; Calcium imaging
类别
资金
- Ministry of Education, Culture, Sports, Scienceand Technology (MEXT) [JP25115002]
- Japan Society for the Promotionof Science (JSPS)
- KAKENHI [JP23220009, JP18H05213, JP19H04899, JP20H03554]
- JSPS KAKENHI [JP17K19445, JP20K22679]
- Core Research for EvolutionalScience and Technology (CREST) program of the Japan Science and TechnologyAgency (JST) [JPMJCR13W1]
- Precursory Research for EmbryonicScience and Technology (PRESTO) program of JST [JPMJPR1684]
- Takeda Science Foundation
- Hokuriku Bank
- Grant for Research Activities of Firstbank of ToyamaScholarship Foundation
- Narishige Neuroscience Research Foundation
- Tamura Science and Technology Foundation
- Research Foundation for Opto-Science and Technology
- BrainScience Foundation
- JSPS [JP18H05213]
General anesthesia can cause retrograde amnesia by suppressing neuronal ensemble activity in the hippocampus. In the experiments, sevoflurane treatment impaired memory and prevented neural activity in the CA1 region. Further analysis revealed a reduction in the reactivation of neuronal ensembles caused by sevoflurane treatment.
General anesthesia could induce amnesia, however the mechanism remains unclear. We hypothesized that suppression of neuronal ensemble activity in the hippocampus by anesthesia during the post-learning period causes retrograde amnesia. To test this hypothesis, two experiments were conducted with sevoflurane anesthesia (2.5%, 30 min): a hippocampus-dependent memory task, the context pre-exposure facilitation effect (CPFE) procedure to measure memory function and in vivo calcium imaging to observe neural activity in hippocampal CA1 during context exploration and sevoflurane/home cage session. Sevoflurane treatment just after context pre-exposure session impaired the CPFE memory, suggesting sevoflurane induced retrograde amnesia. Calcium imaging showed sevoflurane treatment prevented neuronal activity in CA1. Further analysis of neuronal activity with non-negative matrix factorization, which extracts neural ensemble activity based on synchronous activity, showed that sevoflurane treatment reduced the reactivation of neuronal ensembles between during context exploration just before and one day after sevoflurane inhalation. These results suggest that sevoflurane treatment immediately after learning induces amnesia, resulting from suppression of reactivation of neuronal ensembles.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据