期刊
STEM CELLS INTERNATIONAL
卷 2022, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2022/1396735
关键词
-
资金
- Stem Cell and Translation National Key Project [2016YFA0101403]
- National Natural Science Foundation of China [82171250, 81973351]
- Beijing Municipal Natural Science Foundation [5142005]
- Beijing Talents Foundation [2017000021223TD03]
- Support Project of High-level Teachers in Beijing Municipal Universities in the Period of 13th Five-year Plan [TCD20180333]
- Beijing Municipal Health Commission Fund [PXM2020_026283_000005]
- Ten Thousand Talents Fund [2018A03]
- Royal Society-Newton Advanced Fellowship [NA150482]
- Beijing One Hundred, Thousand
- Support Project of High-level Teachers in Beijing Municipal Universities in the Period of 13th Five-year Plan (CIT)
The study successfully reprogrammed human peripheral blood mononuclear cells into induced neural stem cells and tracked the transplantation using GFP gene insertion. The induced neural stem cells and their derivatives survived and differentiated into mature neurons after transplantation.
Neural stem cells (NSCs) and derivatives are potential cellular sources to treat neurological diseases. In the current study, we reprogrammed human peripheral blood mononuclear cells into induced NSCs (iNSCs) and inserted GFP gene into the AAVS1 site for graft tracing. Targeted integration of GFP does not affect the proliferation and differentiation capacity of iNSCs. iNSC-GFP can be further differentiated into dopaminergic precursors (DAPs) and motor neuron precursors (MNPs), respectively. iNSCs were engrafted into the motor cortex and iNSC-DAPs into the striatum and substantia nigra (SN) of a nonhuman primate, respectively. The surviving iNSCs could respond to the microenvironment of the cortex and spontaneously differentiate into mature neurons that extended neurites. iNSC-DAPs survived well and matured into DA neurons following transplantation into the striatum and SN. iNSC-MNPs could also survive and turn into motor neurons after being engrafted into the spinal cord of rats. The results suggest that iNSCs and derivatives have a potential to be used for the treatment of neurological diseases.
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