期刊
JOURNAL OF NANOBIOTECHNOLOGY
卷 20, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12951-022-01687-0
关键词
COVID-19; SARS-CoV-2; Acquired immunity evaluation; Neutralizing antibodies; pGOLD; Surface enhanced fluorescence
资金
- National Natural Science Foundation of China [22274069]
- Shenzhen Science and Technology program project [JCYJ20180504165657443]
- Guangdong Basic and Applied Basic Research Foundation [2022A1515011408]
- Shenzhen San-Ming Project [SZSM201809085]
- Shenzhen Science and Technology Program [JCYJ20210324104007020]
This study developed a platform for the multiplexed evaluation of SARS-CoV-2 neutralizing antibodies against various variants. It was found that individuals recovered from Omicron variant infection showed weak binding strength against non-Omicron variants, while vaccination could enhance antibody avidity more efficiently.
Generated by the immune system post-infection or through vaccination, the effectiveness of antibodies against emerging SARS-CoV-2 variants is crucial for protecting individuals from the COVID-19 pandemic. Herein, a platform for the multiplexed evaluation of SARS-CoV-2 neutralizing antibodies against various variants was designed on the basis of near-infrared (NIR) surface enhanced fluorescence by nano-plasmonic gold chip (pGOLD). Antibody level across variants (Wild-type, Alpha, Beta, Delta, Omicron) was confirmed by the sera from recovered-individuals who were unvaccinated and had infected with Wild-type, Delta, Omicron variants. However, the neutralizing activity against Omicron variant was markedly decreased for individuals infected by Wild-type (similar to 5.6-fold) and Delta variant (similar to 19.1-fold). To the opposite, neutralizing antibody from individuals recovered from Omicron variant infection showed weak binding strength against non-Omicron variants. Antibody evolution over time was studied with individuals 196-530 days post Wild-type infection. Decreasing IgG antibody titer accompanied by increasing IgG binding avidity with elongated post-infection period were observed for the sera from Wild-type recovered-individuals with different post-infection times, suggesting that after the primary infection, a great number of antibodies were generated and then gradually decreased, while the antibody matured over time. By comparing the IgG level of individuals vaccinated I for 27-51 days with individual post-infection, we found that ca. 1 month after two doses of vaccination, the antibody level was comparable to that of 500 days post-infection, and vaccination could enhance IgG avidity more efficiently. This work demonstrated a platform for the multiplexed, high-throughput and rapid screening of acquired immunity against SARS-CoV-2 variants, providing a new approach for the analysis of vaccine effectiveness, immunity against emerging variants, and related serological study.
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