Biocontrol activity of Bacillus halotolerans strain Pl7 against Botryosphaeria dothidea causing apple postharvest decay and potential mechanisms

Apple ring rot, one of the most common apple postharvest diseases during storage, is caused by Botryosphaeria dothidea. Fungicide application is the most widely used method to control this disease, but the increasing environmental and food safety concerns greatly limit their use. The present study aimed to examine the biocontrol activity and underlying action mechanism of Bacillus halotolerans strain Pl7 against B. dothidea. The results revealed that B. halotolerans strain Pl7 exhibited strong inhibitory activity against B. dothidea by 69% in vitro. The culture filtrate of strain Pl7 possessed cellulase, beta-1, 3-glucanase, protease activity and mediated the antifungal activity against B. dothidea. Further analysis demonstrated that culture filtrate of strain Pl7 could cause cell membrane permeabilization of B. dothidea. Apple fruit suffering from ring rot induced by a carbendazim (CBZ)-sensitive or -resistant B. dothidea isolate was much suppressed after being treated with strain Pl7, maintaining postharvest quality. The ability of strain Pl7 to swiftly colonize and thrive in apple fruit wounds was demonstrated by a re-isolation assay. Additional transcriptome studies of untreated and treated apple fruit with strain Pl7 revealed that strain Pl7 mostly changed the expression of genes functioning in plant secondary metabolite biosynthesis and plant-pathogen interaction. In light of these outcomes, the underlying antagonistic mechanism was investigated, and B. halotolerans strain Pl7 was identified as a promsing microbial biocontrol agent against apple postharvest decay.

Automated summary

Apple ring rot, caused by Botryosphaeria dothidea, is a common postharvest disease of apple. This study investigated the biocontrol activity and mechanism of Bacillus halotolerans strain Pl7 against B. dothidea. Results showed that strain Pl7 exhibited strong inhibitory activity against B. dothidea and its culture filtrate possessed antifungal activity. Further analysis revealed that the culture filtrate of strain Pl7 could increase cell membrane permeabilization of B. dothidea. In apple fruit, strain Pl7 suppressed ring rot and showed the ability to colonize and thrive in fruit wounds. Transcriptome studies indicated that strain Pl7 mainly affected genes involved in plant secondary metabolite biosynthesis and plant-pathogen interaction. Overall, B. halotolerans strain Pl7 shows promise as a biocontrol agent against apple postharvest decay.