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Recent advances in recombinase polymerase amplification: Principle, advantages, disadvantages and applications

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2022)

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Article Chemistry, Analytical

Graphene oxide nanosheet-mediated fluorescent RPA turn-on biosensor for rapid RNAi transgenic plant detection

Kai Li et al.

Summary: This paper preliminarily verifies that graphene oxide (GO) nanomaterials enhance the recombinase polymerase amplification (RPA). The GO nanosheets improve the efficiency of RPA amplification by inducing local aggregation. Based on this enhancement effect, an isothermal fluorescence biosensor is developed for detecting RNA interference (RNAi) transgenic plants and exhibits high sensitivity and selectivity. The biosensor provides significant potential for molecular detection.

ANALYTICA CHIMICA ACTA (2022)

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Article Biophysics

Exploiting the orthogonal CRISPR-Cas12a/Cas13a trans-cleavage for dual-gene virus detection using a handheld device

Tian Tian et al.

Summary: This study introduces a highly efficient dual-gene detection technique based on the orthogonal DNA/RNA collateral cleavage mechanism of the Cas12a/Cas13a system. The technique allows simultaneous detection of multiple amplified products and can be implemented on a portable device for accurate infectious disease screening in resource-limited settings.

BIOSENSORS & BIOELECTRONICS (2022)

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Article Biophysics

Rapid and simultaneous visual screening of SARS-CoV-2 and influenza virufses with customized isothermal amplification integrated lateral flow strip

Yong Sun et al.

Summary: This work introduces a new detection technology that can simultaneously test for SARS-CoV-2 and influenza viruses, with advantages such as speed, high sensitivity, no cross-reactivity, and false positives. Its clinical application demonstrates high accuracy, and this portable device is expected to meet the global healthcare system's need for online monitoring of serious infectious diseases.

BIOSENSORS & BIOELECTRONICS (2022)

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Article Infectious Diseases

Development of a recombinase polymerase amplification assay for rapid detection of Streptococcus suis type 2 in nasopharyngeal swab samples

Xiaowu Jiang et al.

Summary: The study developed a rapid molecular diagnostic tool, real-time recombinase polymerase amplification (RT-RPA) assay, for the detection of Streptococcus suis serotype 2 (SS2) infection. The sensitivity and reproducibility of RT-RPA were evaluated and compared with real-time quantitative PCR (RT-qPCR). The results demonstrated that RT-RPA showed high specificity and detection sensitivity, making it a rapid method for SS2 detection.

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE (2022)

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Article Biochemical Research Methods

Establishment of a real-time Recombinase Polymerase Amplification (RPA) for the detection of decapod iridescent virus 1 (DIV1)

Qian-jun Huang et al.

Summary: The study developed a rapid and simple real-time RPA assay for detecting DIV1, which showed high specificity, low detection limit, and good performance in field samples, providing a potential method for on-site diagnosis of DIV1 infection and control of economic losses in the shrimp industry.

JOURNAL OF VIROLOGICAL METHODS (2022)

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Article Biochemical Research Methods

Rapid diagnostic detection of tomato apical stunt viroid based on isothermal reverse transcription-recombinase polymerase amplification

Natalia Kovalskaya et al.

Summary: Two isothermal molecular diagnostic assays based on RT-RPA technology were developed to detect Tomato apical stunt viroid using the AmplifyRP platform, showing distinct specificity and high sensitivity in detecting viroid RNA in plant samples. This research provides a promising method for on-site diagnosis of TASVd in both laboratory and field conditions.

JOURNAL OF VIROLOGICAL METHODS (2022)

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Article Chemistry, Multidisciplinary

An automated nucleic acid detection platform using digital microfluidics with an optimized Cas12a system

Zhen Sun et al.

Summary: This study established an automated and rapid detection platform based on RPA-Cas12a and digital microfluidics for the detection of influenza viruses and SARS-CoV-2. The platform demonstrated high sensitivity, specificity, speed, and automation.

SCIENCE CHINA-CHEMISTRY (2022)

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Article Immunology

Development and Application of Rapid Clinical Visualization Molecular Diagnostic Technology for Cryptococcus neoformans/C. gattii Based on Recombinase Polymerase Amplification Combined With a Lateral Flow Strip

Lei Wang et al.

Summary: Cryptococcus neoformans (C. neoformans)/C. gattii is a fungus that can easily invade the human central nervous system and cause cryptococcal meningitis. Current clinical identification methods have limitations, and this study developed a rapid, specific, and sensitive detection method using the RPA-LFS system to meet clinical demands.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2022)

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Article Chemistry, Analytical

Glycerol Additive Boosts 100-fold Sensitivity Enhancement for One-Pot RPA-CRISPR/Cas12a Assay

Mei Lin et al.

Summary: A glycerol-enhanced one-pot RPA-CRISPR/Cas12a method has been discovered to greatly improve the detection efficiency, making it comparable to the conventional two-step method. The enhanced efficiency is attributed to the phase separation of the RPA and CRISPR/Cas12a system caused by glycerol viscosity.

ANALYTICAL CHEMISTRY (2022)

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Article Agriculture, Multidisciplinary

Rapid and Visual Detection of Barley Yellow Dwarf Virus by Reverse Transcription Recombinase Polymerase Amplification with Lateral Flow Strips

Na-Kyeong Kim et al.

Summary: Barley yellow dwarf virus (BYDV) is a major viral pathogen causing significant losses of cereal crops. In this study, a rapid and reliable method based on isothermal reverse transcription recombinase polymerase amplification (RT-RPA) combined with lateral flow strips (LFS) was established for the detection of BYDV-infected oat samples. The assay showed high specificity, comparable sensitivity to reverse transcription polymerase chain reaction, and successfully detected BYDV in oat samples from different regions and in individual aphids.

PLANT PATHOLOGY JOURNAL (2022)

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Article Chemistry, Analytical

Recombinase polymerase amplification integrated with microfluidics for nucleic acid testing at point of care

Yuemeng Bai et al.

Summary: This review discusses the state-of-the-art of nucleic acid testing (NAT) and its integration with paper-based microfluidics and chip-based microfluidics, providing insights into their advantages and disadvantages. The challenges and potential optimization strategies for microfluidics-based RPA are also addressed.

TALANTA (2022)

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Article Biochemical Research Methods

Rational Design of CRISPR/Cas12a-RPA Based One-Pot COVID-19 Detection with Design of Experiments

Koray Malc et al.

Summary: Simple and effective molecular diagnostic methods are crucial due to the impact of the COVID-19 pandemic. This study utilized statistical design of experiments (DoE) to develop and optimize a CRISPR/Cas12a-RPA-based one-pot detection method, achieving maximum sensitivity and cost efficiency.

ACS SYNTHETIC BIOLOGY (2022)

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Article Biochemical Research Methods

RPA/CRISPR/Cas12a-Based On-Site and Rapid Nucleic Acid Detection of Toxoplasma gondii in the Environment

Rong Lei et al.

Summary: A portable one-pot detection system for Toxoplasma gondii was developed, combining recombinase polymerase amplification (RPA) and CRISPR/Cas12a system. The system showed high selectivity and a limit of detection of 3.3 copies/μL. It has the advantages of rapidness, robustness, and on-site features, making it a promising tool for field applications in remote areas.

ACS SYNTHETIC BIOLOGY (2022)

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Article Immunology

Establishment and Clinical Application of a RPA-LFS Assay for Detection of Capsulated and Non-Capsulated Haemophilus influenzae

Yan Wang et al.

Summary: In this study, a novel method for detecting Haemophilus influenzae was established, which showed high specificity and sensitivity based on specific primers and probes. The practical application results indicated that this method can accurately detect H. influenzae in clinical samples, providing an effective tool for rapid diagnosis of H. influenzae infections.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2022)

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Article Microbiology

Integrated Trinity Test With RPA-CRISPR/Cas12a-Fluorescence for Real-Time Detection of Respiratory Syncytial Virus A or B

Ling Gong et al.

Summary: Respiratory syncytial virus (RSV) is a common virus that causes respiratory infection, especially severe infection in vulnerable populations. Currently, there is no effective vaccine or antiviral treatment for RSV. An integrated trinity test with an RPA-CRISPR/Cas12a-fluorescence (IT-RAISE) assay system was developed to detect RSV A or RSV B infection. This system is simple, rapid, and inexpensive, with high sensitivity and specificity. It has good clinical application prospects and the potential to detect other viral or bacterial infections.

FRONTIERS IN MICROBIOLOGY (2022)

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Review Pathology

Critical insight into recombinase polymerase amplification technology

Mustafa Ahmad Munawar

Summary: Recombinase polymerase amplification (RPA) is a promising technology for rapidly amplifying target nucleic acid from minimally processed samples. This review provides a detailed introduction of RPA molecular mechanism, kits formats, optimization, application, pros, and cons. It also discusses the nonspecificity issue of RPA and questions the novelty of recombinase-aided amplification (RAA).

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS (2022)

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Article Multidisciplinary Sciences

Photocontrolled crRNA activation enables robust CRISPR-Cas12a diagnostics

Menglu Hu et al.

Summary: This article proposes a CRISPR diagnostic method based on optochemical control, which addresses the issues of contamination risks and insufficient sensitivity in nucleic acid amplification-based diagnostics. The method achieves comparable detection sensitivity and specificity to PCR, and avoids contamination risks through photocontrol.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2022)

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Article Plant Sciences

A CRISPR/Cas12a-based portable platform for rapid detection of Leptosphaeria maculans in Brassica crops

Rong Lei et al.

Summary: This study developed a portable diagnostic method for identifying L. maculans, a plant pathogen causing severe phoma stem canker. The method combines CRISPR/Cas12a-based detection and RPA technique, offering high sensitivity and selectivity. Additionally, two portable platforms were developed, enabling on-field detection with flexible portability and simple operation.

FRONTIERS IN PLANT SCIENCE (2022)

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Article Biophysics

Nanozyme-strip for rapid and ultrasensitive nucleic acid detection of SARS-CoV-2

Xiangqin Meng et al.

Summary: The COVID-19 pandemic has led to a high demand for rapid and sensitive detection of SARS-CoV-2. In this study, we developed a method combining RPA and FeS2 nanozyme strips for nucleic acid amplification and colorimetric signal enhancement. The FeS2 nanozymes exhibited high peroxidase-like activity, enabling colorimetric signal amplification using a non-toxic substrate. The method showed a limit of detection for SARS-CoV-2 close to that of RT-PCR, and demonstrated high agreement with previous RT-PCR results for clinical samples. Our findings suggest that nanozyme-based nucleic acid detection has great potential in the development of POCT diagnosis for COVID-19 and other viral infections.

BIOSENSORS & BIOELECTRONICS (2022)

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Article Biochemical Research Methods

Rapid and sensitive recombinase polymerase amplification combined with lateral flow strips for detecting Candida albicans

Fang Wang et al.

Summary: Due to modern lifestyles and increasing medical intervention, clinical infections caused by conditionally pathogenic fungi, especially Candida albicans, are becoming more serious. Recombinase polymerase amplification (RPA) combined with lateral flow strips (LFS) is a promising method for rapid and accurate detection of this pathogenic fungus. However, the simplicity of the method brings the risk of false-positive signals from primer-dimers. This study successfully eliminated primer-dependent artifacts in the RPA reaction by using probes, introducing specific base substitutions, and analyzing primer-probe complexes, leading to an improved RPA-LFS system for specific detection of C. albicans within 25 minutes without interference.

ANALYTICAL BIOCHEMISTRY (2021)

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Article Chemistry, Analytical

Suitcase Lab for Rapid Detection of SARS-CoV-2 Based on Recombinase Polymerase Amplification Assay

Ahmed Abd El Wahed et al.

Summary: A rapid point-of-need detection method for SARS-CoV-2 was developed based on RT-RPA assay, which can detect the virus in approximately 15 minutes. The method was validated in three different laboratories in Africa, showing high accuracy and potential for assisting in detecting COVID-19 cases in low-resource settings to speed up control measures.

ANALYTICAL CHEMISTRY (2021)

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Article Infectious Diseases

Rapid detection of the New Delhi metallo-β-lactamase (NDM) gene by recombinase polymerase amplification

Xiao Wang et al.

Summary: The study established RPA assays for detecting the bla(NDM) gene, which are highly specific, have a short amplification time, and are reliable and promising for clinical diagnosis.

INFECTION GENETICS AND EVOLUTION (2021)

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Article Multidisciplinary Sciences

Development of a reverse transcription recombinase polymerase amplification assay for rapid and direct visual detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)

Yee Ling Lau et al.

Summary: RT-RPA is a rapid and accurate detection method for SARS-CoV-2, with a clinical sensitivity of 98% and specificity of 100%, proving to be a viable alternative to RT-qPCR.

PLOS ONE (2021)

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Article Biochemistry & Molecular Biology

Recombinase Polymerase Amplification Assay with and without Nuclease-Dependent-Labeled Oligonucleotide Probe

Aleksandr Ivanov et al.

Summary: The combination of recombinase polymerase amplification (RPA) and lateral flow test (LFT) is an effective diagnostic tool for rapid pathogen detection in resource-limited conditions. Two methods for generating labeled RPA amplicons were compared, with the primer-labeling approach detecting 10^3 copies of RNA in reaction and having a half-maximal binding concentration 22 times lower than the probe-dependent method. The developed RPA-LFT assay showed successful application for the detection of AMV-infected plants, supporting the use of primer labeling for the detection of other pathogens.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

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Article Chemistry, Multidisciplinary

Digital CRISPR/Cas-Assisted Assay for Rapid and Sensitive Detection of SARS-CoV-2

Joon Soo Park et al.

Summary: The digital-enhanced CRISPR/Cas-assisted one-pot virus detection (deCOViD) is a novel assay that allows qualitative and quantitative detection of SARS-CoV-2 in a very short time with high sensitivity and a broad dynamic range. It outperforms current benchtop-based counterparts and represents one of the fastest and most sensitive CRISPR/Cas-assisted SARS-CoV-2 detection methods to date.

ADVANCED SCIENCE (2021)

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Article Virology

Development of A Super-Sensitive Diagnostic Method for African Swine Fever Using CRISPR Techniques

Meishen Ren et al.

Summary: A nucleic acid detection method using RPA-CRISPR was developed for diagnosing African swine fever, demonstrating high sensitivity and the ability to distinguish ASFV genomic DNA from other porcine viral DNA/RNA without cross-reactivity. The method showed 100% coincidence rate for diagnosing ASFV clinical DNA samples, highlighting its potential for clinical quarantine of ASFV in the swine industry and food security.

VIROLOGICA SINICA (2021)

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Review Chemistry, Analytical

Recent advances in rolling circle amplification-based biosensing strategies-A review

Lulu Xu et al.

Summary: RCA is an efficient enzymatic isothermal reaction using circular probes to amplify circular templates rapidly, with high specificity and the ability to generate customized functional nucleic acids for in situ detection.

ANALYTICA CHIMICA ACTA (2021)

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Article Virology

Early diagnosis of rabies virus infection by RPA-CRISPR techniques in a rat model

Meishen Ren et al.

Summary: A nucleic acid-based assay using RPA-CRISPR was developed for early rabies diagnosis by detecting viral RNA shedding in the cerebrospinal fluid of rats. This method has the potential for clinical diagnosis of human rabies by detecting RABV genomic RNA released from viral particles as early as 3 days postinfection in a rat model.

ARCHIVES OF VIROLOGY (2021)

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Article Biochemical Research Methods

Isothermal recombinase polymerase amplification-lateral flow detection of SARS-CoV-2, the etiological agent of COVID-19

Thomas R. Shelite et al.

Summary: The rapid development of an RPA-LF testing method has shown successful detection of SARS-CoV-2, with high accuracy and potential for use in resource-limited and decentralized laboratories.

JOURNAL OF VIROLOGICAL METHODS (2021)

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Article Medicine, Research & Experimental

One-tube SARS-CoV-2 detection platform based on RT-RPA and CRISPR/Cas12a

Yangyang Sun et al.

Summary: The OR-DETECTR platform for detecting COVID-19 is rapid, accurate, and can be completed in one tube, with a low detection limit and 100% consistency with rRT-PCR results. Additionally, a lateral flow assay based on OR-DETECTR has been developed for COVID-19 detection.

JOURNAL OF TRANSLATIONAL MEDICINE (2021)

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Article Public, Environmental & Occupational Health

Diagnostic Efficacy of Recombinase-Polymerase-Amplification Coupled with Lateral Flow Strip Reading in Patients with Cutaneous Leishmaniasis from the Amazonas Rainforest of Peru

Bruno L. Travi et al.

Summary: Cutaneous leishmaniasis is highly prevalent in rural and sylvatic regions of Latin America, with diagnosis still relying on microscopy in resource-limited areas. Recombinase-polymerase amplification (RPA-LF) shows potential as a point-of-care test, but further simplification and optimization will be needed for broader applicability.

VECTOR-BORNE AND ZOONOTIC DISEASES (2021)

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Article Virology

CRISPR-Cas12a combined with reverse transcription recombinase polymerase amplification for sensitive and specific detection of human norovirus genotype GII.4

Weidong Qian et al.

Summary: This study developed a rapid, reliable, and portable detection system by combining RT-RPA with CRISPR-Cas12a for diagnosing NOV genotype GII.4. The method can be completed within 40 minutes, with a detection limit of up to 9.65 x 10(2)copies/mL and no cross-reactivity with other viruses.

VIROLOGY (2021)

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Article Virology

Visual detection of human metapneumovirus using CRISPR-Cas12a diagnostics

Weidong Qian et al.

Summary: The study developed a detection technique using RT-RPA combined with CRISPR-Cas12a for clinical diagnosis of HmPV. The RT-RPA-Cas12a based fluorescence or LF assay showed high sensitivity and specificity with a detection limit of 6.97 x 10(2) copies/mL, and no cross reaction with other common viruses.

VIRUS RESEARCH (2021)

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Article Chemistry, Analytical

Combined recombinase polymerase amplification/rkDNA-graphene oxide probing system for detection of SARS-CoV-2

Moon Hyeok Choi et al.

Summary: The study combined RPA with an rkDNA-GO probe system for rapid and highly sensitive detection of SARS-CoV-2, exhibiting high selectivity and sensitivity. The total processing time was only 1.6 hours, making it an efficient method for point-of-care detection of SARS-CoV-2.

ANALYTICA CHIMICA ACTA (2021)

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Article Immunology

Rapid Detection of bla KPC, bla NDM, bla OXA-48-like and bla IMP Carbapenemases in Enterobacterales Using Recombinase Polymerase Amplification Combined With Lateral Flow Strip

Fang Wang et al.

Summary: The improved RPA-LFS method allows for rapid and specific detection of CPE in a short period of time, achieving a simple and quick diagnostic purpose.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2021)

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Article Biophysics

Harnessing recombinase polymerase amplification for rapid multi-gene detection of SARS-CoV-2 in resource-limited settings

Dounia Cherkaoui et al.

Summary: The study introduces a rapid, simple, highly sensitive, and multiplexed molecular detection method which simultaneously targets two conserved genes, offering potential advantages and application prospects. The technology demonstrates high specificity for detecting various coronaviruses and shows promise for point-of-care testing.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Microbiology

Rapid and Sensitive Detection of Salmonella spp. Using CRISPR-Cas13a Combined With Recombinase Polymerase Amplification

Bailin An et al.

Summary: The study developed a simple, rapid, and specific detection method for Salmonella spp. using one-tube RPA-Cas13a, while the two-step assay was more sensitive, particularly for samples at low abundance.

FRONTIERS IN MICROBIOLOGY (2021)

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Article Plant Sciences

Recombinase Polymerase Amplification/Cas12a-Based Identification of Xanthomonas arboricola pv. pruni on Peach

Mei Luo et al.

Summary: This study developed a highly sensitive detection method based on RPA/Cas12a, which can specifically detect Xap. The method can produce results within 2 hours and can be performed at 37 degrees Celsius, making it promising for on-site Xap diagnosis and monitoring.

FRONTIERS IN PLANT SCIENCE (2021)

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Article Multidisciplinary Sciences

Rapid detection of Enterococcus and vancomycin resistance using recombinase polymerase amplification

Pimchanok Panpru et al.

Summary: The study established a rapid method for detecting E. faecium and vancomycin-resistance genes, showing 100% sensitivity and specificity in blood and stool samples compared to traditional PCR methods. Additionally, the detection limit of the RPA-LF method was approximately 10 times better than conventional PCR methods.

PEERJ (2021)

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Article Biochemical Research Methods

Rapid and sensitive detection of hepatitis B virus by lateral flow recombinase polymerase amplification assay

Bashan Zhang et al.

Summary: In this study, an LF-RPA assay was developed for rapid detection of HBV, achieving sensitivity down to 10 copies/reaction. The LF-RPA assay proved to be a promising diagnostic tool for quick and accurate HBV detection with high specificity.

JOURNAL OF VIROLOGICAL METHODS (2021)

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Article Parasitology

CRISPR/Cas12a-based on-site diagnostics of Cryptosporidium parvum IId-subtype-family from human and cattle fecal samples

Fuchang Yu et al.

Summary: A new strategy for direct identification of a specific subtype of C. parvum has been successfully developed in this study, allowing for faster and more convenient diagnosis without the need for expensive equipment or highly trained operators.

PARASITES & VECTORS (2021)

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Article Parasitology

Recombinase polymerase amplification with lateral flow strip for detecting Babesia microti infections

Zheng Nie et al.

Summary: The LF-RPA assay targeting B. microti is a new, simple, specific, sensitive, rapid, and convenient method for diagnosing infection with Babesia. Compared to conventional PCR, it has higher sensitivity and faster detection capabilities, making it suitable for field applications and large-scale sample screening.

PARASITOLOGY INTERNATIONAL (2021)

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Article Chemistry, Analytical

Duplex On-Site Detection of Vibrio cholerae and Vibrio vulnificus by Recombinase Polymerase Amplification and Three-Segment Lateral Flow Strips

Pei Wang et al.

Summary: The duplex detection biosensor based on RPA technology and LFS strips showed good specificity and high sensitivity for Vibrio cholerae and Vibrio vulnificus. It is simple and fast, suitable for on-site detection, and capable of simultaneous detection of these two important foodborne bacteria.

BIOSENSORS-BASEL (2021)

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Article Veterinary Sciences

Development and evaluation of recombinase polymerase amplification combined with lateral flow dipstick assays for co-detection of epizootic haemorrhagic disease virus and the Palyam serogroup virus

Zhuo-ran Li et al.

Summary: This study developed a recombinase polymerase amplification combined with lateral flow dipstick (RPA-LFD) assay for the co-detection of EHDV and PALV for the first time. The assay showed high sensitivity and specificity in detecting EHDV and PALV strains, and had no cross-reaction with other orbiviruses. The results demonstrated that RPA-LFD assay is a specific, sensitive, and reliable method that can be applied in the early clinical diagnosis of EHDV and PALV.

BMC VETERINARY RESEARCH (2021)

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Article Biotechnology & Applied Microbiology

Development of a recombinase polymerase amplification combined with lateral flow dipstick assay for rapid and sensitive detection of Heterosigma akashiwo

Bingkun Wang et al.

Summary: A novel technique called RPA-LFD was developed for detecting Heterosigma akashiwo, which demonstrated high specificity and sensitivity. The method is simple, rapid, and allows for direct visualization of results.

JOURNAL OF APPLIED PHYCOLOGY (2021)

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Article Biochemical Research Methods

Sensitively and quickly detecting Vibrio vulnificus by real time recombinase polymerase amplification targeted to vvhA gene

Peng Zhu et al.

Summary: Vibrio vulnificus is a Gram-negative bacterium that can cause serious gastroenteritis or septicemia when people come into contact with seawater or seafood containing the bacteria. The real time recombinase polymerase amplification (real time RPA) method developed in this study showed high specificity and sensitivity in detecting Vibrio vulnificus, making it a potential tool for disease diagnosis and research laboratory applications. The method also demonstrated improved quantitative analysis using second order polynomial regression compared to routine linear regression models.

MOLECULAR AND CELLULAR PROBES (2021)

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Article Multidisciplinary Sciences

Rapid detection of multidrug-resistant tuberculosis based on allele-specific recombinase polymerase amplification and colorimetric detection

Nuntita Singpanomchai et al.

Summary: The study developed a novel allele-specific isothermal recombinase polymerase amplification (AS-RPA) technique for simultaneous detection of drug-resistant gene mutations in tuberculosis, which showed 100% sensitivity and specificity compared to DNA sequencing. This easy-to-use and rapid technology could be potentially integrated for molecular diagnosis of MDR-TB in low-resource settings.

PLOS ONE (2021)

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Article Immunology

A Rapid and Sensitive Detection Method for Pseudomonas aeruginosa Using Visualized Recombinase Polymerase Amplification and Lateral Flow Strip Technology

Haitao Yang et al.

Summary: The study developed a rapid and highly sensitive and specific detection method for Pseudomonas aeruginosa, which showed superior performance in experimental validation, proving to be of great importance in controlling disease spread and development, especially in clinical applications.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2021)

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Article Biochemical Research Methods

Utilization of recombinase polymerase amplification method combined with lateral flow dipstick for visual detection of respiratory syncytial virus

Yu-zhong Xu et al.

MOLECULAR AND CELLULAR PROBES (2020)

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Article Biochemical Research Methods

Recombinase polymerase amplification-lateral flow (RPA-LF) assay combined with immunomagnetic separation for rapid visual detection of Vibrio parahaemolyticus in raw oysters

Wei Jiang et al.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2020)

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Article Biotechnology & Applied Microbiology

Loop-mediated amplification as promising on-site detection approach for Legionella pneumophila and Legionella spp.

Cornelia Reuter et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2020)

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Article Multidisciplinary Sciences

Rapid isothermal duplex real-time recombinase polymerase amplification (RPA) assay for the diagnosis of equine piroplasmosis

Rong Lei et al.

SCIENTIFIC REPORTS (2020)

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Article Infectious Diseases

Development of a recombinase polymerase amplification lateral flow assay for the detection of active Trypanosoma evansi infections

Zeng Li et al.

PLOS NEGLECTED TROPICAL DISEASES (2020)

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Article Food Science & Technology

Recombinase polymerase amplification with polymer flocculation sedimentation for rapid detection of Staphylococcus aureus in food samples

Jinqiang Hu et al.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2020)

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Detection of Plasmodium knowlesi using recombinase polymerase amplification (RPA) combined with SYBR Green I

Meng Yee Lai et al.

ACTA TROPICA (2020)

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Article Chemistry, Analytical

Single universal primer recombinase polymerase amplification-based lateral flow biosensor (SUP-RPA-LFB) for multiplex detection of genetically modified maize

Kai Li et al.

ANALYTICA CHIMICA ACTA (2020)

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Article Parasitology

Application of a recombinase polymerase amplification (RPA) assay and pilot field testing for Giardia duodenalis at Lake Albert, Uganda

Sandra J. Molina-Gonzalez et al.

PARASITES & VECTORS (2020)

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Article Chemistry, Applied

A rapid and convenient method for on-site detection of MON863 maize through real-time fluorescence recombinase polymerase amplification

Xiaofu Wang et al.

FOOD CHEMISTRY (2020)

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Article Biotechnology & Applied Microbiology

Direct detection of methicillin-resistant inStaphylococcusspp. in positive blood culture by isothermal recombinase polymerase amplification combined with lateral flow dipstick assay

Arpasiri Srisrattakarn et al.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY (2020)

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Article Biotechnology & Applied Microbiology

Comparative study of multiplex real-time recombinase polymerase amplification and ISO 11290-1 methods for the detection of Listeria monocytogenes in dairy products

Alejandro Garrido-Maestu et al.

FOOD MICROBIOLOGY (2020)

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Article Veterinary Sciences

Specific Detection and Differentiation Between Brucella melitensis and Brucella abortus by a Duplex Recombinase Polymerase Amplification Assay

M. M. Gumaa et al.

FRONTIERS IN VETERINARY SCIENCE (2020)

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Article Microbiology

A Real-Time Recombinase Polymerase Amplification Method for Rapid Detection of Vibrio vulnificus in Seafood

Xiaohan Yang et al.

FRONTIERS IN MICROBIOLOGY (2020)

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Article Emergency Medicine

Rapid and accurate detection of carbapenem-resistance gene by isothermal amplification in Acinetobacter baumannii

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