期刊
ELIFE
卷 11, 期 -, 页码 -出版社
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.77460
关键词
mitochondria; mitoribosome; antibiotics; translation; Fe-S cluster; aging; Human
类别
资金
- Ragnar Soderbergs stiftelse [M44/16]
- European Research Council [ERC-2018-StG-805230]
- Knut och Alice Wallenbergs Stiftelse [2018.0080]
- EMBO Long-term fellowship [LTF2020-606]
- Karolinska Institutet
- Max Planck Institute for Biology of Ageing
- H2020-MSCA-IF-2017 [799399-Itohribo]
- H2020-MSCA-ITN-2016
The mitoribosome, an important component regulating cellular energy production, is associated with aging. This study reveals the roles of non-protein components in the mitoribosome through analyzing the structure of the small subunit in complex with the anti-tuberculosis drug streptomycin. Findings of iron-sulfur clusters and NAD as fundamental building blocks directly link to mitochondrial disease and aging.
The mitoribosome regulates cellular energy production, and its dysfunction is associated with aging. Inhibition of the mitoribosome can be caused by off-target binding of antimicrobial drugs and was shown to be coupled with a bilateral decreased visual acuity. Previously, we reported mitochondria-specific protein aspects of the mitoribosome, and in this article we present a 2.4-& ANGS; resolution structure of the small subunit in a complex with the anti-tuberculosis drug streptomycin that reveals roles of non-protein components. We found iron-sulfur clusters that are coordinated by different mitoribosomal proteins, nicotinamide adenine dinucleotide (NAD) associated with rRNA insertion, and posttranslational modifications. This is the first evidence of inter-protein coordination of iron-sulfur, and the finding of iron-sulfur clusters and NAD as fundamental building blocks of the mitoribosome directly links to mitochondrial disease and aging. We also report details of streptomycin interactions, suggesting that the mitoribosome-bound streptomycin is likely to be in hydrated gem-diol form and can be subjected to other modifications by the cellular milieu. The presented approach of adding antibiotics to cultured cells can be used to define their native structures in a bound form under more physiological conditions, and since streptomycin is a widely used drug for treatment, the newly resolved features can serve as determinants for targeting.
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