期刊
ELIFE
卷 12, 期 -, 页码 -出版社
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.83077
关键词
lncRNAs; endoderm; SOX17; enhancer; Human
类别
In this study, a previously unannotated lncRNA, T-REX17, was discovered upstream of the SOX17 gene. It was found to be induced following SOX17 activation and its expression was specifically restricted to early definitive endoderm. Loss of T-REX17 affected important functions independent of SOX17 and resulted in an aberrant endodermal transcriptome, signaling pathway deregulation, and defects in epithelial to mesenchymal transition. Consequently, cells lacking T-REX17 were unable to differentiate into more mature endodermal cell types. Overall, this study identified and characterized T-REX17 as a transiently expressed and essential non-coding regulator in early human endoderm differentiation.
Long non-coding RNAs (lncRNAs) have emerged as fundamental regulators in various biological processes, including embryonic development and cellular differentiation. Despite much progress over the past decade, the genome-wide annotation of lncRNAs remains incomplete and many known non-coding loci are still poorly characterized. Here, we report the discovery of a previously unannotated lncRNA that is transcribed 230 kb upstream of the SOX17 gene and located within the same topologically associating domain. We termed it T-REX17 (Transcript Regulating Endoderm and activated by soX17) and show that it is induced following SOX17 activation but its expression is more tightly restricted to early definitive endoderm. Loss of T-REX17 affects crucial functions independent of SOX17 and leads to an aberrant endodermal transcriptome, signaling pathway deregulation and epithelial to mesenchymal transition defects. Consequently, cells lacking the lncRNA cannot further differentiate into more mature endodermal cell types. Taken together, our study identified and characterized T-REX17 as a transiently expressed and essential non-coding regulator in early human endoderm differentiation.
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