期刊
ELIFE
卷 11, 期 -, 页码 -出版社
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.84694
关键词
mitotic chromosome assembly; condensin; kleisin; Xenopus egg extracts; cell cycle regulation; Xenopus
类别
资金
- Japan Society for the Promotion of Science [17K15070, 19H05755, 19K06499, 20H05937, 18H05276, 20H05938, 20K15723]
This study uncovers a mechanism for regulating the cell cycle-specific loading of Condensin I through its kleisin subunit CAP-H N-terminal tail. The results also demonstrate that deletion of the N-terminal tail enables Condensin I to assemble mitotic chromosome-like structures in interphase extracts.
Condensin I is a pentameric protein complex that plays an essential role in mitotic chromosome assembly in eukaryotic cells. Although it has been shown that condensin I loading is mitosis specific, it remains poorly understood how the robust cell cycle regulation of condensin I is achieved. Here, we set up a panel of in vitro assays to demonstrate that cell cycle-specific loading of condensin I is regulated by the N-terminal tail (N-tail) of its kleisin subunit CAP-H. Deletion of the N-tail accelerates condensin I loading and chromosome assembly in Xenopus egg mitotic extracts. Phosphorylation-deficient and phosphorylation-mimetic mutations in the CAP-H N-tail decelerate and accelerate condensin I loading, respectively. Remarkably, deletion of the N-tail enables condensin I to assemble mitotic chromosome-like structures even in interphase extracts. Together with other extract-free functional assays in vitro, our results uncover one of the multilayered mechanisms that ensure cell cycle-specific loading of condensin I onto chromosomes.
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