期刊
ELIFE
卷 11, 期 -, 页码 -出版社
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.62087
关键词
cell polarity; Caco-2 cells; polarity proteins; STED; human intestine; Human; Mouse
类别
资金
- Agence Nationale de la Recherche [ANR-10-INBS-04, ANR-17-CE14-0032, ANR-11-LABX-0054, ANR-11-IDEX-0001-02]
- Instituts thematiques multiorganismes Plan Cancer
- Ligue Contre le Cancer Post doctoral grant
- Agence Nationale de la Recherche (ANR) [ANR-17-CE14-0032] Funding Source: Agence Nationale de la Recherche (ANR)
Epithelial tissues acquire their integrity and function through apico-basal polarization of cells, where proteins of the PAR and Crumbs complexes play pivotal roles. By imaging endogenous proteins, we identified the regionalized and segregated organization of PAR and Crumbs complexes at epithelial apical junctions.
Epithelial tissues acquire their integrity and function through the apico-basal polarization of their constituent cells. Proteins of the PAR and Crumbs complexes are pivotal to epithelial polarization, but the mechanistic understanding of polarization is challenging to reach, largely because numerous potential interactions between these proteins and others have been found, without a clear hierarchy in importance. We identify the regionalized and segregated organization of members of the PAR and Crumbs complexes at epithelial apical junctions by imaging endogenous proteins using stimulated-emission-depletion microscopy on Caco-2 cells, and human and murine intestinal samples. Proteins organize in submicrometric clusters, with PAR3 overlapping with the tight junction (TJ) while PALS1-PATJ and aPKC-PAR6 beta form segregated clusters that are apical of the TJ and present in an alternated pattern related to actin organization. CRB3A is also apical of the TJ and partially overlaps with other polarity proteins. Of the numerous potential interactions identified between polarity proteins, only PALS1-PATJ and aPKC-PAR6 beta are spatially relevant in the junctional area of mature epithelial cells, simplifying our view of how polarity proteins could cooperate to drive and maintain cell polarity.
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