Toxicokinetics of a Single Oral Dose of OTA on Dezhou Male Donkeys

Ochratoxin (OTA) is widely present in a wide range of foods and feeds, causing adverse effects on animals and humans. This study aims to explore the toxicokinetics of OTA-contaminated materials on the Dezhou male donkey. Donkeys received a single orally dose of 2500 mu g OTA/kg BW, obtained from Aspergillus ochraceus culture material. The concentrations of OTA in plasma collected at 0, 5, 10, 15, 20, 30, 45 min, and at 1, 1.5, 2, 3, 6, 9, 12, 24, 48, 72, 96 and 120 h were detected by HPLC. OTA eliminated in urine and feces were quantified at 6-h intervals up to 24 h and then at 4-h intervals up to 120 h. The results suggested that the maximum concentration of OTA in plasma was observed at 12 h after administration, with a mean value of 10.34 mu g/mL. The total excretion in both urine and feces was about 10% of the intake until 120 h.

Automated summary

Ochratoxin (OTA) is found in various foods and feeds, causing harmful effects on animals and humans. This study investigates the toxicokinetics of OTA-contaminated materials in Dezhou male donkeys. Donkeys were orally administered a single dose of 2500 μg OTA/kg BW from Aspergillus ochraceus culture material. The concentration of OTA in plasma was measured at different time points, and the excretion of OTA in urine and feces was quantified. The results showed that the maximum concentration of OTA in plasma was observed at 12 hours after administration, with an average value of 10.34 μg/mL. The total excretion in urine and feces accounted for approximately 10% of the intake within 120 hours.