Evaluation of the Adsorption Efficacy of Bentonite on Aflatoxin M1 Levels in Contaminated Milk

The existence of aflatoxin M-1 (AFM(1)) in raw milk results in economic losses and public health risks. This research aims to examine the capability of bentonite to adsorb and/or eliminate AFM(1) from various raw milk types. In addition, the effects of numerous bentonites (HAFR 1, 2, 3 and 4) on the nutritional characteristics of the milk were studied. Our findings revealed that goat milk had the highest value of AFM(1) (490.30 ng/L) in comparison to other milks. AFM(1) adsorption was influenced by applying bentonite (0.5 and 1 g) in a concentration-dependent manner for different time intervals (from 0 to 12 h). The percentage of AFM(1) reached the maximum adsorption level after 12 h to 100, 98.5 and 98% for bentonites HAFR 3, 1 and 2, respectively. HAFR 3 (1 g bentonite) presented higher adsorption efficiency than other bentonites used in the phosphate buffer saline (PBS) and milk. Residual levels of AFM(1) reached their lowest values of 0 and 1.5 ng/L while using HAFR 3 in PBS and milk, respectively. With regard to the influence of bentonite on the nutritional characteristics of milk, there was an increase in fat, protein and solid non-fat ratio while using HAFR 3 and 4, yet decreased lactose in comparison with the control. Scanning Electron Microscopy and Fourier Transform-Infrared Spectroscopy both identified bentonites as superior AFM(1) binders. The results demonstrated that bentonite, particularly HAFR 3, was the most effective adsorbent and could thus be a promising candidate for the decontamination of AFM(1) in milk.

Automated summary

The aim of this research was to investigate the ability of bentonite to adsorb and/or eliminate aflatoxin M-1 (AFM(1)) from different types of raw milk. The study found that goat milk had the highest AFM(1) value compared to other milks. The adsorption of AFM(1) by bentonite was found to be concentration-dependent and reached maximum levels after 12 hours. Bentonite HAFR 3 exhibited the highest adsorption efficiency and resulted in the lowest residual levels of AFM(1) in both phosphate buffer saline and milk. Furthermore, the use of bentonite led to changes in the nutritional characteristics of milk.