Development of Polydiphenylamine@Electrochemically Reduced Graphene Oxide Electrode for the D-Penicillamine Sensor from Human Blood Serum Samples Using Amperometry

D-penicillamine (PA) is a sulfur group-containing drug prescribed for various health issues, but overdoses have adverse effects. Therefore, regular, selective, and sensitive sensing is essential to reduce the need for further treatment. In this study, diphenylamine (DPA) was electropolymerized in an aqueous acidic medium. The PA detection sensitivity, selectivity, and limit of detection were enhanced by electropolymerizing DPA on an electrochemically reduced graphene oxide (ERGO)/glassy carbon (GC) surface. The formation of p-DPA and ERGO was investigated using various techniques. The as-prepared p-DPA@ERGO/GC revealed the excellent redox-active (N-C to N=C) sites of p-DPA. The p-DPA@ERGO/GC electrode exhibited excellent electrochemical sensing ability towards PA determination because of the presence of the -NH-functional moiety and effective interactions with the -SH group of PA. The p-DPA@ERGO/GC exhibited a high surface coverage of 9.23 x 10(-12) mol cm(-2). The polymer-modified p-DPA@ERGO/GC electrode revealed the amperometric determination of PA concentration from the 1.4 to 541 mu M wide range and the detection limit of 0.10 mu M. The real-time feasibility of the developed p-DPA@ERGO/GC electrode was tested with a realistic PA finding in human blood serum samples and yielded a good recovery of 97.5-101.0%, confirming the potential suitability in bio-clinical applications.

Automated summary

In this study, diphenylamine was electropolymerized on an electrochemically reduced graphene oxide/glass carbon surface, enhancing the sensitivity, selectivity, and detection limit for D-penicillamine detection. The developed electrode showed excellent electrochemical sensing ability for D-penicillamine determination and demonstrated potential suitability in bio-clinical applications.