Rac1 controls cell turnover and reversibility of the involution process in postpartum mammary glands

Cell turnover in adult tissues is essential for maintaining tissue homeostasis over a life span and for inducing the morphological changes associated with the reproductive cycle. However, the underlying mechanisms that coordinate the balance of cell death and proliferation remain unsolved. Using the mammary gland, we have discovered that Rac1 acts as a nexus to control cell turnover. Postlactational tissue regression is characterised by the death of milk secreting alveoli, but the process is reversible within the first 48 h if feeding recommences. In mice lacking epithelial Rac1, alveolar regression was delayed. This defect did not result from failed cell death but rather increased cell turnover. Fitter progenitor cells inappropriately divided, regenerating the alveoli, but cell death also concomitantly accelerated. We discovered that progenitor cell hyperproliferation was linked to nonautonomous effects of Rac1 deletion on the macrophageal niche with heightened inflammation. Moreover, loss of Rac1 impaired cell death with autophagy but switched the cell death route to apoptosis. Finally, mammary gland reversibility failed in the absence of Rac1 as the alveoli failed to recommence lactation upon resuckling.

Automated summary

Cell turnover is vital for tissue homeostasis and reproductive cycle, and Rac1 controls cell turnover in the mammary gland. Lack of Rac1 delayed alveolar regression, resulting in increased cell turnover. Hyperproliferation of progenitor cells and accelerated cell death were observed in the absence of Rac1, linked to macrophageal inflammation and impaired autophagic cell death. Mammary gland reversibility was also impaired without Rac1, leading to failure of lactation upon resuckling.