Development of molecular inversion probes for soybean progeny genomic selection genotyping

Increasing rate of genetic gain for key agronomic traits through genomic selection requires the development of new molecular methods to run genome-wide single-nucleotide polymorphisms (SNPs). The main limitation of current methods is the cost is too high to screen breeding populations. Molecular inversion probes (MIPs) are a targeted genotyping-by-sequencing (GBS) method that could be used for soybean [Glycine max (L.) Merr.] that is both cost-effective, high-throughput, and provides high data quality to screen breeder's germplasm for genomic selection. A 1K MIP SNP set was developed for soybean with uniformly distributed markers across the genome. The SNPs were selected to maximize the number of informative markers in germplasm being tested in soybean breeding programs located in the northern-central and middle-southern regions of the United States. The 1K SNP MIP set was tested on diverse germplasm and a recombinant inbred line (RIL) population. Targeted sequencing with MIPs obtained an 85% enrichment for the targeted SNPs. The MIP genotyping accuracy was 93% overall, whereas homozygous call accuracy was 98% with <10% missing data. The accuracy of MIPs combined with its low per-sample cost makes it a powerful tool to enable genomic selection within soybean breeding programs.

Automated summary

The increasing rate of genetic gain for agronomic traits in farming requires the development of new molecular tools for genome-wide screening. The cost of current methods limits their use in screening breeding populations. Molecular inversion probes (MIPs) are a cost-effective and high-throughput genotyping method that can be used for soybean breeding programs. A set of 1K MIP SNPs for soybean was developed and tested on diverse germplasm, showing high genotyping accuracy and low cost. This makes MIPs a powerful tool for genomic selection in soybean breeding programs.