期刊
JOURNAL OF PLANT PHYSIOLOGY
卷 204, 期 -, 页码 27-35出版社
ELSEVIER GMBH
DOI: 10.1016/j.jplph.2016.07.010
关键词
Tomato; Agrobacterium rhizogenes rolB gene; Suppression subtractive hybridization; Photosynthesis
资金
- Ministero dell'Universita e della Ricerca, Progetto Nazionale di Ricerca Biotecnologie Avanzate
- Ministero delle Politiche Agricole e Forestali, project Protezione delle piante mediante l'uso di marcatori molecolari (PROMAR)
- Progetto Ateneo Sapienza University di Roma
Insertion of Agrobacterium rhizogenes rolB gene into plant genome affects plant development, hormone balance and defence. However, beside the current research, the overall transcriptional response and gene expression of rolB as a modulator in plant is unknown. Transformed rolB tomato plant (Solanum lycopersicum L.) cultivar Tondino has been used to investigate the differential expression profile. Tomato is a well-known model organism both at the genetic and molecular level, and one of the most important commercial food crops in the world. Through the construction and characterization of a cDNA subtracted library, we have investigated the differential gene expression between transgenic clones of rolB and control tomato and have evaluated genes specifically transcribed in transgenic rolB plants. Among the selected genes, five genes encoding for chlorophyll a/b binding protein, carbonic anhydrase, cytochrome b(6)/f complex Fe-S subunit, potassium efflux antiporter 3, and chloroplast small heat-shock protein, all involved in chloroplast function, were identified. Measurement of photosynthesis efficiency by the level of three different photosynthetic parameters (F-v/F-m, rETR, NPQ) showed rolB significant increase in non-photochemical quenching and a, b chlorophyll content. Our results point to highlight the role of rolB on plant fitness by improving photosynthesis. (C) 2016 Elsevier GmbH. All rights reserved.
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