期刊
JOURNAL OF PLANT PHYSIOLOGY
卷 195, 期 -, 页码 50-58出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.jplph.2016.02.018
关键词
Hydrogen-rich water; Nitric oxide (NO); NO synthase (NOS); Nitrate reductase (NR); NR genes; Enzyme
资金
- National Natural Science Foundation of China [31160398, 31560563]
- Post Doctoral Foundation of China [20100470887, 2012T50828]
- Key Project of Chinese Ministry of Education [211182]
- Research Fund for the Doctoral Program of Higher Education [20116202120005]
- Natural Science Foundation of Gansu Province, China [1308RJZA179]
Hydrogen gas (H-2) is involved in plant development and stress responses. Cucumber explants were used to study whether nitric oxide (NO) is involved in H-2-induced adventitious root development. The results revealed that 50% and 100% hydrogen-rich water (HRW) apparently promoted the development of adventitious root in cucumber. While, the responses of HRW-induced adventitious rooting were blocked by a specific NO scavenger 2-(4-carboxypheny1)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide potassium salt (cPTIO), NO synthase (NOS) enzyme inhibitor NG-nitro-L-arginine methylester hydrochloride (L-NAME) and nitrate reductase (NR) inhibitor NaN3. HRW also increased NO content and NOS and NR activity both in a dose-and time-dependent fashion. Moreover, molecular evidence showed that HRW up regulated NR genes expression in explants. The results indicate the importance of NOS and NR enzymes, which might be responsible for NO production in explants during H2-induced root organogenesis. Additionally, peroxidase (POD) and indoleacetic acid oxidase (IAAO) activity was significantly decreased in the explants treated with HRW, while HRW treatment significantly increased polyphenol oxidase (PPO) activity. In addition, cPTIO, L-NAME and NaN3 inhibited the actions of HRW on the activity of these enzymes. Together, NO may be involved in H-2-induced adventitious rooting, and NO may be acting downstream in plant H-2 signaling cascade. 2016 Elsevier GmbH. All rights reserved.
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