Exogenous 3,3′-diindolylmethane increases Brassica napus L. seedling shoot growth through modulation of superoxide and hydrogen peroxide content

Brassica napus L. (cv. AV Garnet) seeds were pre-treated with 15 mu M 3,3'-diindolylmethane (DIM) to investigate whether DIM could enhance seed germination. Further treatment of seedlings with 15 mu M DIM for 14 days explored the effects on seedling shoot growth. Exogenous DIM led to improved germination percentage, increased seedling shoot lengths, and increased fresh and dry weights. Furthermore, DIM triggered induction of superoxide radical (O-2(-)) and hydrogen peroxide (H2O2) content however, no change in malondialdehyde (MDA) content and cell death (assessed with Evans Blue assay) was detected for both the control and DIM treated seedling shoots. We also observed increases in superoxide dismutase (SOD) activity and ascorbate peroxidase (APX) activity in response to exogenous DIM, two fundamental enzymes in the control of reactive oxygen species (ROS) in plants. These results indicate that exogenous DIM treatment enhances seed germination and improves seedling shoot growth through possible activation of a reactive oxygen species signalling pathway involving O-2(-) and H2O2 in B. napus. (C) 2016 Elsevier GmbH. All rights reserved.