期刊
G3-GENES GENOMES GENETICS
卷 13, 期 3, 页码 -出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/g3journal/jkad005
关键词
ADE17; SHM2; CHO2; cell size; nuclear division; phosphatidylcholine
Enzymes involved in one-carbon metabolism play crucial roles in regulating cell division. Loss of these enzymes leads to abnormalities in cell size and cell cycle kinetics, affecting cell proliferation. Loss of Ade17p specifically delays the START phase, while loss of Shm2p reduces growth rate. Furthermore, the inability to synthesize phosphatidylcholine in cho2 Delta cells delays START and decreases nuclear elongation coherence, accompanied by metabolite changes.
Enzymes of one-carbon (1C) metabolism play pivotal roles in proliferating cells. They are involved in the metabolism of amino acids, nucleotides, and lipids and the supply of all cellular methylations. However, there is limited information about how these enzymes are regulated during cell division and how cell cycle kinetics are affected in several loss-of-function mutants of 1C metabolism. Here, we report that the levels of the S. cerevisiae enzymes Ade17p and Cho2p, involved in the de novo synthesis of purines and phosphatidylcholine (PC), respectively, are cell cycle-regulated. Cells lacking Ade17p, Cho2p, or Shm2p (an enzyme that supplies 1C units from serine) have distinct alterations in size homeostasis and cell cycle kinetics. Loss of Ade17p leads to a specific delay at START, when cells commit to a new round of cell division, while loss of Shm2p has broader effects, reducing growth rate. Furthermore, the inability to synthesize PC de novo in cho2 Delta cells delays START and reduces the coherence of nuclear elongation late in the cell cycle. Loss of Cho2p also leads to profound metabolite changes. Besides the expected changes in the lipidome, cho2 Delta cells have reduced levels of amino acids, resembling cells shifted to poorer media. These results reveal the different ways that 1C metabolism allocates resources to affect cell proliferation at multiple cell cycle transitions.
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