期刊
ECOLOGY AND EVOLUTION
卷 12, 期 12, 页码 -出版社
WILEY
DOI: 10.1002/ece3.9639
关键词
animal model; GWAS; heritability; SNP density; wild population
资金
- H2020 European Research Council
- Scottish Natural Heritage
- Wellcome Trust
- Natural Environment Research Council
- European Research Council [NE/S007407/1]
- Medical Research Council
- [MC_UU_00007/1]
Understanding the genetic architecture of quantitative traits in wild populations is crucial for understanding trait evolution. Marker density affects the reliability of relatedness estimates and the detection of quantitative trait loci. In this study on Soay sheep, high-density SNP data revealed additional SNP-trait associations and confirmed previously identified QTL. Simultaneously fitting fixed and random effects in GWAS is more powerful than pre-correcting for covariates in a separate model.
Understanding the genetic architecture underpinning quantitative traits in wild populations is pivotal to understanding the processes behind trait evolution. The 'animal model' is a popular method for estimating quantitative genetic parameters such as heritability and genetic correlation and involves fitting an estimate of relatedness between individuals in the study population. Genotypes at genome-wide markers can be used to estimate relatedness; however, relatedness estimates vary with marker density, potentially affecting results. Increasing density of markers is also expected to increase the power to detect quantitative trait loci (QTL). In order to understand how the density of genetic markers affects the results of quantitative genetic analyses, we estimated heritability and performed genome-wide association studies (GWAS) on five body size traits in an unmanaged population of Soay sheep using two different SNP densities: a dataset of 37,037 genotyped SNPs and an imputed dataset of 417,373 SNPs. Heritability estimates did not differ between the two SNP densities, but the high-density imputed SNP dataset revealed four new SNP-trait associations that were not found with the lower density dataset, as well as confirming all previously-found QTL. We also demonstrated that fitting fixed and random effects in the same step as performing GWAS is a more powerful approach than pre-correcting for covariates in a separate model.
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