期刊
CELL REPORTS
卷 41, 期 11, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.celrep.2022.111820
关键词
-
类别
资金
- NIH
- [R35 NS111596]
This study identified a distinctive release-dependent form of synaptic facilitation, which dynamically amplifies multi-vesicular release and requires neuronal contact with astrocytes and astrocytic glutamate uptake.
Synaptic facilitation is a major form of short-term plasticity typically driven by an increase in residual presyn-aptic calcium. Using near-total internal reflection fluorescence (near-TIRF) imaging of single vesicle release in cultured hippocampal synapses, we demonstrate a distinctive, release-dependent form of facilitation in which probability of vesicle release is higher following a successful glutamate release event than following a failure. This phenomenon has an onset of <= 500 ms and lasts several seconds, resulting in clusters of successful release events. The release-dependent facilitation requires neuronal contact with astrocytes and astrocytic glutamate uptake by EAAT1. It is not observed in neurons grown alone or in the presence of astrocyte-conditioned media. This form of facilitation dynamically amplifies multi-vesicular release. Facilitation-evoked release events exhibit spatial clustering and have a preferential localization toward the active zone center. These results uncover a rapid astrocyte-dependent form of facilitation acting via modulation of multi-vesicular release and displaying distinctive spatiotemporal properties.
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