4.7 Article

Repurposing the Endogenous CRISPR-Cas9 System for High- Efficiency Genome Editing in Lacticaseibacillus paracasei

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Microbiology

Extracellular vesicles of Lacticaseibacillus paracasei PC-H1 induce colorectal cancer cells apoptosis via PDK1/AKT/Bcl-2 signaling pathway

Yangqian Shi et al.

Summary: Extracellular vesicles derived from Lacticaseibacillus paracasei inhibit the growth of colorectal cancer cells and induce apoptosis through the PDK1/AKT/Bcl-2 signaling pathway. This research provides new insights into the anti-tumor effects of probiotics and opens up new opportunities for the treatment of colon cancer.

MICROBIOLOGICAL RESEARCH (2022)

Article Plant Sciences

Highly efficient genome editing in Xanthomonas oryzae pv. oryzae through repurposing the endogenous type I-C CRISPR-Cas system

Dandan Jiang et al.

Summary: Efficient genome editing using an identified type I-C CRISPR-Cas system in Xoo allows for precise modifications of the genome, providing a valuable tool for functional genomics studies of rice bacterial blight and potentially other CRISPR-harboring bacterial plant pathogens.

MOLECULAR PLANT PATHOLOGY (2022)

Article Multidisciplinary Sciences

Genomic and epigenetic landscapes drive CRISPR-based genome editing in Bifidobacterium

Meichen Pan et al.

Summary: This study repurposed the endogenous type I-G CRISPR-Cas system and an exogenous CRISPR base editor for genome engineering in B. animalis subsp. lactis, showcasing the impact of genomic and epigenetic contexts on editing outcomes across strains. Additionally, a CRISPR-cytosine base editor was optimized to sensitize multiple B. lactis strains to tetracycline by introducing C.G-to-T.A mutations.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2022)

Article Biotechnology & Applied Microbiology

Portable CRISPR-Cas9(N) System for Flexible Genome Engineering in Lactobacillus acidophilus, Lactobacillus gasseri, and Lactobacillus paracasei

Yong Jun Goh et al.

Summary: This study developed a CRISPR-based editing system for genome manipulations in three Lactobacillus species, demonstrating its efficacy in two distantly related Lactobacillus species. This versatile Cas9 system facilitates genome engineering compared to conventional gene replacement systems and is a valuable gene editing modality in species without native CRISPR-Cas systems. Overall, this portable tool expands the genome editing toolbox for studying health-promoting mechanisms and engineering beneficial microbes.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2021)

Article Biotechnology & Applied Microbiology

High-Efficiency Genome Editing Based on Endogenous CRISPR-Cas System Enhances Cell Growth and Lactic Acid Production in Pediococcus acidilactici

Ling Liu et al.

Summary: This study developed a high-efficiency endogenous CRISPR-Cas system-based genome editing tool for Pediococcus acidilactici, allowing for various genetic manipulations. The engineered strain edited by this tool showed significant advantages in cell growth and lactic acid fermentation, making it a sophisticated chassis species for synthetic biology and bioindustry.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2021)

Review Food Science & Technology

Health-Promoting Properties of Lacticaseibacillus paracasei: A Focus on Kefir Isolates and Exopolysaccharide-Producing Strains

Ana Agustina Bengoa et al.

Summary: Recent reports have focused on the health-promoting properties of Lacticaseibacillus paracasei isolated from kefir and water kefir, even though it is not the predominant species in these fermented beverages. The study discusses the importance of selecting an adequate method for identifying L. paracasei to avoid mislabeling in products and publications.
Review Biochemistry & Molecular Biology

Complex Oligosaccharide Utilization Pathways in Lactobacillus

Manuel Zuniga et al.

Summary: This review summarizes the current knowledge on the catabolic pathways of complex carbohydrates utilized by lactobacilli at biochemical and genetic levels, highlighting their essential traits for survival in competitive environments.

CURRENT ISSUES IN MOLECULAR BIOLOGY (2021)

Review Microbiology

Evolutionary classification of CRISPR-Cas systems: a burst of class 2 and derived variants

Kira S. Makarova et al.

NATURE REVIEWS MICROBIOLOGY (2020)

Article Microbiology

Occurrence and Diversity of CRISPR Loci in Lactobacillus casei Group

Lan Yang et al.

FRONTIERS IN MICROBIOLOGY (2020)

Article Food Science & Technology

Modulatory properties of Lactobacillus paracasei fermented milks on gastric inflammatory conditions

Ana A. Bengoa et al.

INTERNATIONAL DAIRY JOURNAL (2020)

Article Multidisciplinary Sciences

Genome editing using the endogenous type I CRISPR-Cas system in Lactobacillus crispatus

Claudio Hidalgo-Cantabrana et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2019)

Article Biotechnology & Applied Microbiology

CRISPR/Cas9-Assisted Seamless Genome Editing in Lactobacillus plantarum and Its Application in N-Acetylglucosamine Production

Ding Zhou et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2019)

Article Biotechnology & Applied Microbiology

Using the Endogenous CRISPR-Cas System of Heliobacterium modesticaldum To Delete the Photochemical Reaction Center Core Subunit Gene

Patricia L. Baker et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2019)

Article Biochemical Research Methods

High-Level dCas9 Expression Induces Abnormal Cell Morphology in Escherichia coli

Suhyung Cho et al.

ACS SYNTHETIC BIOLOGY (2018)

Article Multidisciplinary Sciences

Characterizing the activity of abundant, diverse and active CRISPR-Cas systems in lactobacilli

Alexandra B. Crawley et al.

SCIENTIFIC REPORTS (2018)

Review Microbiology

The Lactobacillus casei Group: History and Health Related Applications

Daragh Hill et al.

FRONTIERS IN MICROBIOLOGY (2018)

Article Genetics & Heredity

CRISPRdisco: An Automated Pipeline for the Discovery and Analysis of CRISPR-Cas Systems

Alexandra R. Crawley et al.

CRISPR JOURNAL (2018)

Article Biochemistry & Molecular Biology

Harnessing the native type I-B CRISPR-Cas for genome editing in a polyploid archaeon

Feiyue Cheng et al.

JOURNAL OF GENETICS AND GENOMICS (2017)

Review Biochemistry & Molecular Biology

Deciphering, Communicating, and Engineering the CRISPR PAM

Ryan T. Leenay et al.

JOURNAL OF MOLECULAR BIOLOGY (2017)

Article Biotechnology & Applied Microbiology

A versatile one-step CRISPR-Cas9 based approach to plasmid-curing

Ida Lauritsen et al.

MICROBIAL CELL FACTORIES (2017)

Article Microbiology

A decade of discovery: CRISPR functions and applications

Rodolphe Barrangou et al.

NATURE MICROBIOLOGY (2017)

Article Biotechnology & Applied Microbiology

CRISPR-Cas9D10A Nickase-Assisted Genome Editing in Lactobacillus casei

Xin Song et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2017)

Article Biochemistry & Molecular Biology

Harnessing Type I and Type III CRISPR-Cas systems for genome editing

Yingjun Li et al.

NUCLEIC ACIDS RESEARCH (2016)

Review Biology

CRISPR-Cas: biology, mechanisms and relevance

Frank Hille et al.

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES (2016)

Article Biotechnology & Applied Microbiology

Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

Yu Jiang et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2015)

Article Microbiology

Occurrence and activity of a type II CRISPR-Cas system in Lactobacillus gasseri

Rosemary Sanozky-Dawes et al.

MICROBIOLOGY-SGM (2015)

Article Biochemistry & Molecular Biology

Guide RNA Functional Modules Direct Cas9 Activity and Orthogonality

Alexandra E. Briner et al.

MOLECULAR CELL (2014)

Review Biotechnology & Applied Microbiology

CRISPR-Cas systems for editing, regulating and targeting genomes

Jeffry D. Sander et al.

NATURE BIOTECHNOLOGY (2014)

Article Biochemistry & Molecular Biology

CRISPR-Cas9-assisted recombineering in Lactobacillus reuteri

Jee-Hwan Oh et al.

NUCLEIC ACIDS RESEARCH (2014)

Article Biotechnology & Applied Microbiology

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

Wenyan Jiang et al.

NATURE BIOTECHNOLOGY (2013)

Article Biochemistry & Molecular Biology

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

James E. DiCarlo et al.

NUCLEIC ACIDS RESEARCH (2013)

Article Biochemistry & Molecular Biology

CRISPRTarget: Bioinformatic prediction and analysis of crRNA targets

Ambarish Biswas et al.

RNA BIOLOGY (2013)

Article Multidisciplinary Sciences

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

Martin Jinek et al.

SCIENCE (2012)

Article Biochemistry & Molecular Biology

WebLogo: A sequence logo generator

GE Crooks et al.

GENOME RESEARCH (2004)