期刊
STEM CELL RESEARCH & THERAPY
卷 13, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13287-022-03203-5
关键词
Tendon; Human pluripotent stem cells; Tenocytes; Tendon regeneration
资金
- Japan Society for the Promotion of Science [21H02643]
- AMED [18bm0704024h0001]
The study established an hPSC-derived MKX-tdTomato reporter cell line and developed an efficient induction method. MKX-tdTomato(+) cells expressed tendon-specific markers and can be used for studying the development or regeneration of tendon tissue.
Tendon regeneration is difficult because detailed knowledge about tendon progenitor cells (TPCs), which produce tenocytes to repair tendon tissue, has not been revealed. Mohawk homeobox (MKX) is a marker of TPCs or tenocytes, but a human pluripotent stem cell (hPSC)-based reporter system that visualizes MKX+ cells has not been developed. Here, we established an hPSC-derived MKX-tdTomato reporter cell line and tested the induction ratio of MKX-tdTomato(+) cells using our stepwise/xeno-free differentiation protocol. MKX-tdTomato(+) cells were generated with high efficiency and expressed tendon-specific markers, including MKX, SCX, TNMD, and COL1A1. Our MKX-tdTomato hPSC line would be a useful tool for studying the development or regeneration of tendon tissue.
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