In vitro study of the modulatory effects of heat-killed bacterial biomass on aquaculture bacterioplankton communities

Recent studies have shown that the addition of non-viable microbial biomass or their components (postbiotics) to fish feed can modulate the gut microbiome and positively influence fish health in aquaculture systems. However, no information was hitherto available on the use of non-viable microbial biomass to manipulate aquaculture bacterioplankton communities. To fill this gap, here we used an in vitro model to assess the effects of heat-killed biomasses of an antagonistic strain Pseudoalteromonas rubra SubTr2 and a non-antagonist strain Escherichia coli DH5 alpha on bacterioplankton communities of a recirculating aquaculture system (RAS). Our results showed that these biomasses can have generalist and species-specific effects on aquaculture bacterioplankton structure and function. In addition, they enriched the abundance of bacterial predators, reduced bacterial load and potentially influenced nutrient cycling and pathogen development in aquaculture water. Despite its preliminary nature, for the first time, this study showed that heat-killed microbial biomass has potential application as an in situ modulator of bacterioplankton in aquaculture systems.

Automated summary

Recent studies have shown that the addition of non-viable microbial biomass to fish feed can positively influence fish health in aquaculture systems. This study assessed the effects of heat-killed biomasses on bacterioplankton communities in a recirculating aquaculture system and found that they can have generalist and species-specific effects, enrich bacterial predators, reduce bacterial load, and potentially influence nutrient cycling and pathogen development in aquaculture water.