Proteomics analysis in myocardium of spontaneously hypertensive rats

Hypertension-related left ventricular hypertrophy is recognized as a good predictor of adverse cardiovascular events. However, the underlying mechanism of left ventricular hypertrophy is still not fully understood. This study employed liquid chromatography coupled with tandem mass spectrometry to investigate global changes in protein profile in myocardium of spontaneously hypertensive rat, a classical animal model of essential hypertension. There were 369 differentially expressed proteins in myocardium between spontaneously hypertensive rats and normotensive rats. Xenobiotic catabolic process, cholesterol binding and mitochondrial proton-transporting ATP synthase were found to be the most significantly enriched biological process, molecular function and cellular component terms of Gene Ontology, respectively. Drug metabolism-cytochrome P450 was revealed to be the most significantly enriched Kyoto Encyclopedia of Genes and Genomes pathways. FYN proto-oncogene, Src family tyrosine kinase was found to have the most interactions with other proteins. Differentially expressed proteins involved in xenobiotic catabolic process, lipid transport and metabolism, mitochondrial function might be targets for further study of hypertension-related left ventricular hypertrophy.

Automated summary

This study investigated global changes in protein profile in the myocardium of spontaneously hypertensive rats using liquid chromatography coupled with tandem mass spectrometry. There were 369 differentially expressed proteins in the myocardium between spontaneously hypertensive rats and normotensive rats. These differentially expressed proteins were involved in important pathways related to biological processes, molecular functions, and cellular components, and the drug metabolism-cytochrome P450 pathway was the most significantly enriched. FYN proto-oncogene, Src family tyrosine kinase had the highest number of interactions with other proteins. These differentially expressed proteins may serve as targets for further study of hypertension-related left ventricular hypertrophy.