期刊
JOURNAL OF PHYSIOLOGY-LONDON
卷 594, 期 14, 页码 4051-4070出版社
WILEY-BLACKWELL
DOI: 10.1113/JP271681
关键词
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资金
- National Health and Medical Research Council of Australia [APP1023460, APP1041582, 634501]
- Australian Research Council
- National Heart Foundation of Australia [PF 11P 6024]
- National Health and Medical Research Council [APP1039101, APP1059516]
Genetic mutations in cardiac troponin I (cTnI) account for 5% of families with hypertrophic cardiomyopathy. Hypertrophic cardiomyopathy is associated with disorganization of cytoskeletal proteins and altered energy metabolism. The L-type Ca2+ channel (ICa-L) plays an important role in regulating mitochondrial function. This involves a functional communication between the channel and mitochondria via the cytoskeletal network. We investigate the role of ICa-L in regulating mitochondrial function in 25- to 30-week-old cardiomyopathicmice expressing the human disease-causing mutation Gly203Ser in cTnI (cTnI-G203S). The inactivation rate of ICa-L is significantly faster in cTnI-G203S myocytes [cTnI-G203S: tau(1) = 40.68 +/- 3.22, n = 10 vs. wild-type (wt): tau(1) = 59.05 +/- 6.40, n = 6, P < 0.05]. Activation of ICa-L caused a greater increase in mitochondrialmembrane potential (Psi(m), 29.19 +/- 1.85%, n = 15 vs. wt: 18.84 +/- 2.01%, n = 10, P < 0.05) and metabolic activity (24.40 +/- 6.46%, n = 8 vs. wt: 9.98 +/- 1.57%, n = 9, P < 0.05). The responses occurred because of impaired communication between ICa-L and F-actin, involving lack of dynamic movement of actin-myosin and block of the mitochondrial voltage-dependent anion channel. Similar responses were observed in precardiomyopathic mice. ICa-L antagonists nisoldipine and diltiazemdecreased Psi(m) to basal levels. We conclude that the Gly203Ser mutation leads to impaired functional communication between ICa-L and mitochondria, resulting in a 'hypermetabolic' state. This might contribute to development of cTnI-G203S cardiomyopathy because the response is present in young precardiomyopathic mice. ICa-L antagonists might be effective in reducing the cardiomyopathy by altering mitochondrial function.
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