期刊
CELL CALCIUM
卷 58, 期 3, 页码 246-253出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ceca.2015.06.002
关键词
Heart; Ca2+ spark; Ryanodine receptor; Ca2+-induced Ca2+ release; Confocal microscopy
类别
资金
- National Institutes of Health [R01HL057832, R01AR054098]
- Schweppe Foundation
- Loyola University Chicago
Single ryanodine receptor (RyR) Ca2+ flux amplitude (i(Ca-RyR)) decreases as intra-sarcoplasmic reticulum (SR) Ca2+ levels fall during a cardiac Ca2+ spark. Since i(Ca-RyR) drives the inter-RyR Ca2+-induced Ca2+ release (CICR) that underlies the spark, decreasing i(Ca-RyR) may contribute to spark termination because RyRs that spontaneously close may stay closed. To test this possibility, we simultaneously measured local cytosolic and intra-SR ([Ca2+](cyto) and [Ca2+](SR)) during Ca2+ sparks in permeabilized rabbit ventricular myocytes. Local cytosolic or intra-SR Ca2+ dynamics were manipulated using Ca2+ buffers. Buffer manipulations applied in cells had no effect on individual RyR channels reconstituted in planar lipid bilayers. Presence of a fast cytosolic Ca2+ buffer (BAPTA) significantly suppressed Ca2+ spark activity and sparks terminated earlier at a higher than usual [Ca2+](SR) level (similar to 80% vs. similar to 62%). When cytosolic Ca2+ buffer power was reduced (i.e. cytosolic EGTA level decreased), sparks terminated later and at a lower than usual [Ca2+](SR) level (similar to 45% vs. similar to 62%). When intra-SR Ca2+ buffer power was increased, sparks also terminated later and at a lower than usual [Ca2+](SR) (similar to 48% vs. similar to 62%). These results suggest that cytosolic local control of inter-RyR CICR by i(Ca-RyR) plays a substantial role during the spark termination process. Thus, alterations in local cytosolic Ca2+ handling dynamics in the dyadic cleft (Ca2+ buffering, extrusion, etc.) likely influence Ca2+ spark termination. (C) 2015 Elsevier Ltd. All rights reserved.
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