期刊
CELL CALCIUM
卷 57, 期 5-6, 页码 321-336出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ceca.2015.02.003
关键词
Pacing; Electrophysiology; Calcium; Ion channels; Mitochondria; Sarcoplasmic reticulum; Rat neonatal cardiomyocytes; Cardiomyocytes derived from human; induced pluripotent stem cells; Genetically engineered fluorescent probes
类别
资金
- NIH [R01 HL15162, R01 HL107600]
- BMBF [01GN0824]
The quintessential property of developing cardiomyocytes is their ability to beat spontaneously. The mechanisms underlying spontaneous beating in developing cardiomyocytes are thought to resemble those of adult heart, but have not been directly tested. Contributions of sarcoplasmic and mitochondrial Ca2+-signaling vs. I-f-channel in initiating spontaneous beating were tested in human induced Pluripotent Stem cell-derived cardiomyocytes (hiPS-CM) and rat Neonatal cardiomyocytes (rN-CM). Whole-cell and perforated-patch voltage-clamping and 2-D confocal imaging showed: (1) both cell types beat spontaneously (60-140/min, at 24 degrees C); (2) holding potentials between -70 and 0 mV had no significant effects on spontaneous pacing, but suppressed action potential formation; (3) spontaneous pacing at 50 mV activated cytosolic Ca2+-transients, accompanied by in-phase inward current oscillations that were suppressed by Na+-Ca2+-exchanger (NCX)- and ryanodine receptor (RyR2)-blockers, but not by Ca2+- and If-channels blockers; (4) spreading fluorescence images of cytosolic Ca2+-transients emanated repeatedly from preferred central cellular locations during spontaneous beating; (5) mitochondrial un-coupler, FCCP at non-depolarizing concentrations (similar to 50 nM), reversibly suppressed spontaneous pacing; (6) genetically encoded mitochondrial Ca2+-biosensor (mitycam-E31Q) detected regionally diverse, and FCCP-sensitive mitochondrial Ca2+-uptake and release signals activating during I-NCX oscillations; (7) I-f-channel was absent in rN-CM, but activated only negative to 80 mV in hiPS-CM; nevertheless blockers of If-channel failed to alter spontaneous pacing. (C) 2015 Elsevier Ltd. All rights reserved.
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