期刊
NUTRIENTS
卷 15, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/nu15010093
关键词
Tenebrio molitor; atopic dermatitis; MAPK; trypsin hydrolysate
This study investigated the effects of Tenebrio molitor trypsin hydrolysate (TMTH) as an anti-atopic dermatitis (AD) agent and its underlying mechanism in a DNCB-induced AD mouse model. The findings showed that TMTH significantly reduced mast cell infiltration, dermal and epidermal thickness, and serum IgE levels. Furthermore, TMTH suppressed the MyD88-dependent MAPK signaling pathway. Therefore, TMTH has potential as a treatment for AD.
Atopic dermatitis (AD) is a widely researched chronic inflammatory skin disease with a complex etiology. The increased prevalence of AD necessitates exploration of natural sources as potential therapeutic agents with limited side effects. In the current study, a 1-chloro-2,4-dinitrobenzene (DNCB)-induced AD mouse model was used to examine the anti-AD effects of Tenebrio molitor trypsin hydrolysate (TMTH) and its underlying molecular mechanism. DNCB-treated mice were treated with TMTH (1 and 10 mg/kg), and prednisolone (3 mg/kg) was used as the positive control. Serum and skin tissue samples were collected for subsequent analyses. The expression levels of proteins linked to the myeloid differentiation primary response 88 (MyD88)-dependent mitogen-activated protein kinase (MAPK) signaling pathway and serum IgE levels were estimated via Western blotting technique and ELISA (enzyme-linked immunosorbent assay), respectively. Inflammatory cell infiltration and thickening of the dorsal skin were measured using toluidine blue and hematoxylin and eosin staining, respectively. Oral administration of TMTH significantly reduced mast cell infiltration and dermal and epidermal thickness. Moreover, TMTH treatment reduced serum IgE levels. Western blotting confirmed that TMTH treatment suppressed the MyD88-dependent MAPK signaling pathway. Therefore, TMTH substantially inhibited AD-like skin lesion formation via immunomodulation, showing considerable potential for AD treatment.
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