4.7 Article

Altered Gastric Microbiota and Inflammatory Cytokine Responses in Patients with Helicobacter pylori-Negative Gastric Cancer

期刊

NUTRIENTS
卷 14, 期 23, 页码 -

出版社

MDPI
DOI: 10.3390/nu14234981

关键词

stomach neoplasms; gastric microbiota; 16S rRNA; cytokines; interleukin-1beta

资金

  1. National Research Foundation of Korea (NRF) - Korea government (MSIT) [2020R1G1A1010927]
  2. Korean College of Helicobacter and Upper Gastrointestinal Research Foundation Grant [KCHUGR-202101001]
  3. VA [IK2CX001717]
  4. National Research Foundation of Korea [2020R1G1A1010927] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)

向作者/读者索取更多资源

This study investigated the microbial changes and host inflammatory cytokine responses in H. pylori-negative gastric cancer patients. The results showed that the gastric mucosal microbiota in GC patients had reduced diversity and a different composition compared to controls. Lacticaseibacillus was enriched, while Haemophilus and Campylobacter were depleted in the cancer group. Furthermore, the combined abundance of these taxa correlated significantly with gastric mucosal IL1B expression.
The role of the gastric mucosal microbiome in Helicobacter pylori-negative gastric cancer (GC) remains unclear. Therefore, we aimed to characterize the microbial alterations and host inflammatory cytokine responses in H. pylori-negative GC. Gastric mucosal samples were obtained from 137 H. pylori-negative patients with GC (n = 45) and controls (chronic gastritis or intestinal metaplasia, n = 92). We performed 16S rRNA gene sequencing (n = 67), a quantitative reverse transcription-polymerase chain reaction to determine the relative mRNA expression levels of TNF (tumor necrosis factor), IL1B (interleukin 1 beta), IL6 (interleukin 6), CXCL8 (C-X-C motif chemokine ligand 8), IL10 (interleukin 10), IL17A (interleukin 17A), TGFB1 (transforming growth factor beta 1) (n = 113), and the correlation analysis between sequencing and expression data (n = 47). Gastric mucosal microbiota in patients with GC showed reduced diversity and a significantly different composition compared to that of the controls. Lacticaseibacillus was significantly enriched, while Haemophilus and Campylobacter were depleted in the cancer group compared to the control group. These taxa could distinguish the two groups in a random forest algorithm. Moreover, the combined relative abundance of these taxa, a GC microbiome index, significantly correlated with gastric mucosal IL1B expression, which was elevated in the cancer group. Overall, altered gastric mucosal microbiota was found to be associated with increased mucosal IL1B expression in H. pylori-negative GC.

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