期刊
MATERIALS
卷 15, 期 24, 页码 -出版社
MDPI
DOI: 10.3390/ma15248802
关键词
collagen; hydroxyapatite composite; acidic-gelatin; alternate immersion method; hydroxyapatite precipitation; basic-fibroblast growth factor; bone formation; bone histology; bone substitute materials; dental implantology
Bone-substitute materials play a crucial role in dental implantology. This study explores the use of collagen/hydroxyapatite/acidic gelatin/basic fibroblast growth factor constructs to enhance bone formation. Animal experiments demonstrate that the constructs moderately increase bone formation in cranial bone defects. Immersion time of 20 minutes leads to the greatest bone formation.
Bone-substitute materials are essential in dental implantology. We prepared collagen (Col)/hydroxyapatite (Hap)/acidic gelatin (AG)/basic fibroblast growth factor (b-FGF) constructs with enhanced bone-forming capability. The Col/Hap apatite composites were prepared by immersing Col sponges alternately in calcium and phosphate ion solutions five times, for 20 and 60 min, respectively. Then, the sponges were heated to 56 degrees C for 48 h. Scanning electron microscopy/energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectroscopy, and X-ray diffraction analyses showed that the Col/Hap composites contained poorly crystalline Hap precipitates on the Col matrix. Col/Hap composite granules were infiltrated by AG, freeze-dried, and immersed in b-FGF solution. The wet quaternary constructs were implanted in rat cranial bone defects for 8 weeks, followed by soft X-ray measurements and histological analysis. Animal studies have shown that the constructs moderately increase bone formation in cranial bone defects. We found that an alternate immersion time of 20 min led to the greatest bone formation (p < 0.05). Constructs placed inside defects slightly extend the preexisting bone from the defect edges and lead to the formation of small island-like bones inside the defect, followed by disappearance of the constructs. The combined use of Col, Hap, AG, and b-FGF might bring about novel bone-forming biomaterials.
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