期刊
ARABIAN JOURNAL OF CHEMISTRY
卷 15, 期 12, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.arabjc.2022.104369
关键词
Adynerin; Bioavailability; Oleandrin; Pharmacokinetics; UPLC-MS; MS
This study investigated the pharmacokinetic differences of oleandrin and adynerin in rats and found variations in their bioavailability.
Oleandrin and adynerin are the main toxic components of oleander, an evergreen shrub or a small tree of the oleander family, which belongs to the class of cardiac glycosides exhibiting delayed action. The pharmacokinetic differences of oleandrin and adynerin in rats were studied by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) under two different administration modes: oral (5 mg/kg) and sublingual intravenous injection (1 mg/ kg). The chromatographic column was UPLC BEH C18 (50 mm x 2.1 mm, 1.7 lm), and the col-umn temperature was set at 40 degrees C. The mobile phase was acetonitrile-water (containing 0.1 % for-mic acid), with gradient elution, the flow rate was 0.4 mL/min, and the elution time was 4 min. Electrospray (ESI) positive ion mode detection with multiple reaction monitoring mode (MRM) was used for quantitative analysis: oleandrin m/z 577-> 145, adynerin m/z 534-> 113, and internal standard m/z 237-> 135. The established UPLC-MS/MS method was successfully applied to the pharmacokinetics in rats after administering oleandrin and adynerin. The bioavailability of olean-drin and adynerin was found to be low, 7.0 % and 93.1 %; respectively.(c) 2022 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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