期刊
NATURE COMMUNICATIONS
卷 13, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41467-022-35242-0
关键词
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资金
- National Natural Science Foundation of China [81970245, 81370241, 82270357, 81770432]
- Innovation Capacity Support Project of Shaanxi Province in China [2019PT-23, 2022YWZX-PG-01]
- Science and Technology Project of Shaanxi Province in China [2019ZDLSF04-03-01]
- Scientific Research Project of Shaanxi Administration of Traditional Chinese Medicine [2019-GJ-JC012, 2022-SLRH-YQ-004, 2021-04-ZZ-001, 2021-QYPT-003]
- Special Foundation for Talents of Northwest AF University [2452017335]
Deficiency of TRPC1 and TRPC6 channels ameliorates LPS-induced heart failure, prolongs survival, and suppresses cardiac inflammation and ER Ca2+ release.
Intracellular Ca2+ dysregulation is a key marker in septic cardiac dysfunction; however, regulation of the classic Ca2+ regulatory modules cannot successfully abolish this symptom. Here we show that the knockout of transient receptor potential canonical (TRPC) channel isoforms TRPC1 and TRPC6 can ameliorate LPS-challenged heart failure and prolong survival in mice. The LPS-triggered Ca2+ release from the endoplasmic reticulum both in cardiomyocytes and macrophages is significantly inhibited by Trpc1 or Trpc6 knockout. Meanwhile, TRPC's molecular partner - calmodulin - is uncoupled during Trpc1 or Trpc6 deficiency and binds to TLR4's Pococurante site and atypical isoleucine-glutamine-like motif to block the inflammation cascade. Blocking the C-terminal CaM/IP3R binding domain in TRPC with chemical inhibitor could obstruct the Ca2+ leak and TLR4-mediated inflammation burst, demonstrating a cardioprotective effect in endotoxemia and polymicrobial sepsis. Our findings provide insight into the pathogenesis of endotoxemic cardiac dysfunction and suggest a novel approach for its treatment. TRPCs, nonselective cation channels, are involved in cardiac contraction and conduction. Here, the authors show that Trpc1/6 deficiency or pharmacological inhibition improves endotoxemic cardiac dysfunction and prolongs survival by prominently suppressing cardiac inflammation and ER Ca2+ release.
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