期刊
NATURE COMMUNICATIONS
卷 13, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41467-022-35131-6
关键词
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资金
- Canadian Foundation for Innovation
- Canadian Institutes of Health Research (CIHR) [MOP 43998, FDN154327]
- Tier 1 Canada Research Chair (2006-2020)
- Cystic Fibrosis Canada
- Hospital for Sick Children Foundation Student Scholarship Program
- GlycoNet Summer Awards Program for Undergraduate Students
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Canada Excellence Research Chairs Program (L. Mahal CERC in Glycomics)
- Netherlands Organization for Scientific Research (NWO-Vidi)
This study reveals the molecular mechanism of synthase-dependent secretion systems in producing exopolysaccharides, providing insights into the role of Pseudomonas aeruginosa in lung infections.
Synthase-dependent secretion systems are a conserved mechanism for producing exopolysaccharides in Gram-negative bacteria. Although widely studied, it is not well understood how these systems are organized to coordinate polymer biosynthesis, modification, and export across both membranes and the peptidoglycan. To investigate how synthase-dependent secretion systems produce polymer at a molecular level, we determined the crystal structure of the AlgK-AlgX (AlgKX) complex involved in Pseudomonas aeruginosa alginate exopolysaccharide acetylation and export. We demonstrate that AlgKX directly binds alginate oligosaccharides and that formation of the complex is vital for polymer production and biofilm attachment. Finally, we propose a structural model for the AlgEKX outer membrane modification and secretion complex. Together, our study provides insight into how alginate biosynthesis proteins coordinate production of a key exopolysaccharide involved in establishing persistent Pseudomonas lung infections.
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