4.8 Article

Identification of visual cortex cell types and species differences using single-cell RNA sequencing

期刊

NATURE COMMUNICATIONS
卷 13, 期 1, 页码 -

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NATURE PORTFOLIO
DOI: 10.1038/s41467-022-34590-1

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资金

  1. Natural Science Foundation of China [81870682, 81961128021, 82201231]
  2. National Key R&D Program of China [2018YFA0108300, 2022YEF0203200, 2021YFF1200900, 2021YFF1200903]
  3. Guangdong Provincial Key RD Programs [2018B030335001, 2018B030337001]
  4. Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program [2017BT01S138]
  5. Science and Technology Program of Guangzhou [202007030011, 202007030010]
  6. China Postdoctoral Science Foundation [2022M713609, 2019M663256]
  7. Major Project on Brain Science and Brain-Like Computing of the Ministry of Science and Technology of China [2021ZD0200103]
  8. Self-supporting Program of Guangzhou Laboratory [ZL-SRPG2200305, ZL-SRPG2200702]
  9. Wellcome Sanger core funding [WT206194]

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The study describes a single-cell RNA sequencing dataset of macaque V1 cortex cells, revealing similarities and specific markers compared to human cortex. Differences in gene expression profiles and neuron diversity across species were found, providing insights into cognitive functions.
The laminar organization of macaque V1 cortex is similar to that seen in humans. Here the authors describe a single-cell RNA sequencing dataset of macaque V1 cortex cells. The primate neocortex exerts high cognitive ability and strong information processing capacity. Here, we establish a single-cell RNA sequencing dataset of 133,454 macaque visual cortical cells. It covers major cortical cell classes including 25 excitatory neuron types, 37 inhibitory neuron types and all glial cell types. We identified layer-specific markers including HPCAL1 and NXPH4, and also identified two cell types, an NPY-expressing excitatory neuron type that expresses the dopamine receptor D3 gene; and a primate specific activity-dependent OSTN + sensory neuron type. Comparisons of our dataset with humans and mice show that the gene expression profiles differ between species in relation to genes that are implicated in the synaptic plasticity and neuromodulation of excitatory neurons. The comparisons also revealed that glutamatergic neurons may be more diverse across species than GABAergic neurons and non-neuronal cells. These findings pave the way for understanding how the primary cortex fulfills the high-cognitive functions.

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