期刊
JOURNAL OF PHYSICS D-APPLIED PHYSICS
卷 49, 期 37, 页码 -出版社
IOP PUBLISHING LTD
DOI: 10.1088/0022-3727/49/37/375401
关键词
transepithelial electrical resistance; electrical impedance spectroscopy; interdigitated electrodes; microfluidics; transwell; geometric correction factor
资金
- CIBER-BBN
- CSIC [PIE-201450E116]
- Ministerio de Economia y Competitividad [SAF2014-62114-EXP, DPI2015-65401-C3-3-R]
- Instituto de Salud Carlos III
Transepithelial electrical resistance (TEER) measurements are regularly used in in vitro models to quantitatively evaluate the cell barrier function. Although it would be expected that TEER values obtained with the same cell type and experimental setup were comparable, values reported in the literature show a large dispersion for unclear reasons. This work highlights a possible error in a widely used formula to calculate the TEER, in which it may be erroneously assumed that the entire cell culture area contributes equally to the measurement. In this study, we have numerically calculated this error in some cell cultures previously reported. In particular, we evidence that some TEER measurements resulted in errors when measuring low TEERs, especially when using Transwell inserts 12 mm in diameter or microfluidic systems that have small chamber heights. To correct this error, we propose the use of a geometric correction factor (GCF) for calculating the TEER. In addition, we describe a simple method to determine the GCF of a particular measurement system, so that it can be applied retrospectively. We have also experimentally validated an interdigitated electrodes (IDE) configuration where the entire cell culture area contributes equally to the measurement, and it also implements minimal electrode coverage so that the cells can be visualized alongside TEER analysis.
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