期刊
JOURNAL OF FUNCTIONAL FOODS
卷 99, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jff.2022.105329
关键词
Metagenome; Short -chain fatty acid; Gut microbiota; Fructooligosaccharides; Galactooligosaccharides; In vitro fermentation
资金
- Heilongjiang Significant Special Project of Engineering Science and Technology [2019ZX06B02]
- fund of the Scientific Research Starting Foundation for the Doctoral Program [XDB2017-12]
- Innovative Talents Project [CXRC2017010]
- Heilongjiang Bayi Agricultural University
This study investigated the fermentation characteristics of fructooligosaccharides (FOS) and galactooligosaccharides (GOS) with infant fecal inoculum. The results showed that FOS and GOS significantly influenced the composition and metabolism of the intestinal microbiota, leading to increased production of short-chain fatty acids (SCFAs) and lactate.
Oligosaccharides are widely added to infant formula milk powders as prebiotics for intestinal health. Using in vitro fermentation conditions, this study investigated the fermentation characteristics of fructooligosaccharides (FOS) and galactooligosaccharides (GOS) with infant fecal inoculum. The changes in short-chain fatty acids (SCFAs) and lactate production were monitored by HPLC. The contents of SCFAs and lactate increased significantly in the GOS and FOS + GOS groups. Furthermore, the metagenomic analysis revealed that microbial communities were distinctively different. Most bacteria were significantly positively correlated with SCFAs production and negatively correlated with pH, particularly Bifidobacterium, Enterococcus, Weissella and Streptococcus. More importantly, the relative abundance of most carbohydrate-active enzymes (CAZymes, EC:2.3.1.54, EC:2.3.1.9 and EC:2.8.3.8, etc.) were significantly higher in the GOS and FOS + GOS groups. These results suggested that FOS and GOS had a significant regulatory effect on the composition and metabolism of the intestinal microbiota, further promoting the production of SCFAs and lactate.
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