The flavonoids extract from Okra flowers protects against DSS-induced colitis via regulating NF-κB signaling pathway and gut microbiota

Okra flower is an untapped flavonoid-rich resource, and flavonoids from it (AFE) have been demonstrated intestinal protective potential due to anti-colorectal cancer properties, but other intestinal-related effects remain to be explored. Here, we found that AFE, as a safe natural product, effectively alleviate 2.5% dextran sulfate sodium (DSS)-induced intestinal inflammation in C57BL/6N and DSS-induced injury of human intestinal epithelial cells NCM460. AFE maintained colon integrity by promoting the levels of tight junction proteins, ZO-1 and Occludin, and modulating the NF-kappa B signaling pathway to prevent further tissue damage. Meanwhile, gut microbiota was involved in the protective effect of AFE in vivo, with Akkermansia making a major contribution. Moreover, AFE exhibited similar inhibitory effects on the NF-kappa B pathway in vitro and exerted antioxidant effects as a ROS scavenger by regulating the Nrf2 signaling pathway. In summary, this study demonstrated the potential of AFE in restoring intestinal health in vitro and in vivo.

Automated summary

Okra flower is a flavonoid-rich resource and its flavonoids (AFE) have potential in preventing colorectal cancer. This study found that AFE effectively alleviated intestinal inflammation and injury, maintained colon integrity, and involved gut microbiota in the protective effect. AFE also exhibited inhibitory effects on the NF-kappa B pathway and antioxidant effects through regulating Nrf2 signaling pathway.