期刊
JOURNAL OF NATURAL FIBERS
卷 19, 期 15, 页码 11114-11123出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15440478.2021.2009404
关键词
Antheraea pernyi silk fibroin; degumming; silk biomaterials; molecular weight; mechanical properties; degradation behavior
资金
- Hubei Provincial Natural Science Foundation of China [2020CFB646]
- National Natural Science Foundation of China [31600774]
This study investigated the effects of different degumming methods on the properties of regenerated Antheraea pernyi silk fibroin biomaterials. It was found that silk fibroin prepared by NaHCO3 degumming had a higher molecular weight, leading to improved mechanical properties of ASF film, while Na2CO3 degumming produced more low molecular weight silk fibroin.
The extraction of fibroin (degumming) from the silk fibers impacts the properties of silk fibroin. However, the effect of the degumming method on structures and properties of regenerated Antheraea pernyi silk fibroin (ASF) biomaterials has not been investigated in detail. In this study, Na2CO3 and NaHCO3 are selected to remove sericin of Antheraea pernyi silks. The results show that the silk fibroin prepared by NaHCO3 degumming possessed a higher molecular weight, demonstrated by SDS-PAGE and viscosity testing. Na2CO3 degumming produced more low molecular weight silk fibroin protein below 40 kDa, whereas NaHCO3 processing produced more silk fibroin protein above 70 kDa. The increased molecular weight significantly improved the mechanical properties of ASF film. The enzyme degradation results indicated that the degradation rate of ASF film prepared by Na2CO3 degumming was faster in the early stage due to the presence of low molecular weight silk fibroin. After 21 days, the two silk fibroin films showed approximate degradation ratios. These results showed that the degumming conditions have a significant effect on the structure and properties of ASF biomaterials, and provide options for regulating the mechanical properties and degradation rate of ASF biomaterials.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据