Development and Characterization of SSR Markers via de Novo Transcriptome Assembly in Apocynum. hendersonii

To provide a theoretical and practical foundation for the genetic analysis of Apocynum species, SSRs were identified based on the transcriptomic data of A.hendersonii leaves. A total of 50,957 unigenes containing 5 660 SSR motifs with an average of 1 SSR locus per 9.09 Kb were identified. The dinucleotide repeat motifs were the most prevalent, having 48.23% of the total repeat, with the dominant repeats being AG/CT (1,703) followed by A/T (1,222). Furthermore, the results of genetic relationship analsyis based on the allele polymorphism of the selected SSR primer showed that A. hendersonii and A.venetum grouped into various clades, and those from different regions clusted into different subgroups.These identified SSR markers may provide a foundation for genetic identification, diversity analysis and marker-assisted genetic breeding in Apocynum species.

Automated summary

SSRs were identified in A.hendersonii leaves based on transcriptomic data, resulting in the discovery of 50,957 unigenes containing 5,660 SSR motifs. The most prevalent repeat motifs were dinucleotides, with AG/CT and A/T being the dominant repeats. Genetic relationship analysis showed A.hendersonii and A.venetum grouped into different clades, with regional variations in subgroup clustering. These identified SSR markers have potential applications in genetic identification, diversity analysis, and marker-assisted breeding in Apocynum species.