期刊
VIRUSES-BASEL
卷 15, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/v15020426
关键词
SARS-CoV-2; neutralizing antibodies; anti-spike antibody; sVNT; eCLIA; cVNT
类别
Vaccination against COVID-19 is crucial in fighting the pandemic. The sVNT test, with high specificity and reliability, can be a reliable complement to routine anti-S serological assays.
Vaccination against COVID-19 is the main public health approach to fight against the pandemic. The Spike (S) glycoprotein of SARS-CoV-2 is the principal target of the neutralizing humoral response. We evaluated the analytical and clinical performances of a surrogate virus neutralization test (sVNT) compared to conventional neutralization tests (cVNTs) and anti-S eCLIA assays in recovered and/or vaccinated healthcare workers. Our results indicate that sVNTs displayed high specificity and no cross-reactivity. Both eCLIA and sVNT immunoassays were good at identifying cVNT serum dilutions >= 1:16. The optimal thresholds when identifying cVNT titers >= 1:16, were 74.5 U/mL and 49.4 IU/mL for anti-S eCLIA and sVNT, respectively. Our data show that neutralizing antibody titers (Nab) differ from one individual to another and may diminish over time. Specific assays such as sVNTs could offer a reliable complementary tool to routine anti-S serological assays.
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