期刊
VIRUSES-BASEL
卷 15, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/v15020369
关键词
aryl hydrocarbon receptor; Junin virus; arenavirus; Argentine hemorrhagic fever; host therapeutic target
类别
This study analyzed the effect of pharmacological modulation of aryl hydrocarbon receptor (AHR) on Junin virus (JUNV) infection, and found that the activation or depression of AHR signaling pathway may play a role in the outcome of human viral infections. Treatment with CH223191, a specific AHR antagonist, significantly inhibited JUNV infection in vitro. These findings suggest the potential of AHR as a therapeutic target for controlling viral infections.
Junin virus (JUNV), a member of the family Arenaviridae, is the etiological agent of the Argentine hemorrhagic fever, an endemic disease in the rural region of Argentina lacking a specific chemotherapy. Aryl hydrocarbon receptor (AHR) is expressed in several mammalian tissues and has been indicated as a sensor of ligands from variable sources and a modulator of the cell immune response. Interestingly, recent studies have suggested that the activation or depression of the AHR signaling pathway may play a role in the outcome of diverse human viral infections. In the present report, the effect of the pharmacological modulation of AHR on JUNV in vitro infection was analyzed. An initial microarray screening showed that the AHR pathway was overexpressed in JUNV-infected hepatic cells. Concomitantly, the infection of Vero and Huh-7 cells with the JUNV strains IV4454 and Candid#1 was significantly inhibited in a dose-dependent manner by treatment with CH223191, a specific AHR antagonist, as detected by infectivity assays, real-time RT-PCR and immunofluorescence detection of viral proteins. Furthermore, the pro-viral role of AHR in JUNV infection appears to be independent of the IFN-I pathway. Our findings support the promising perspectives of the pharmacological modulation of AHR as a potential target for the control of AHF.
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