4.6 Article

Proteomic analysis of small extracellular vesicles from the plasma of patients with hepatocellular carcinoma

期刊

WORLD JOURNAL OF SURGICAL ONCOLOGY
卷 20, 期 1, 页码 -

出版社

BMC
DOI: 10.1186/s12957-022-02849-y

关键词

Proteomics; Extracellular vesicles; Hepatocellular carcinoma; Complement

资金

  1. Natural Science Foundation of Guangxi
  2. Central Government Guiding Local Scientific and Technological Development by Guangxi Science and Technology Department [2020GXNSFDA238006]
  3. Medical High Level Talents Training Plan in Guangxi [ZY21195024]
  4. Science and Technology Planned Project in Guilin [G202002005]
  5. Guangxi Distinguished Experts Special Fund [20210102-1]
  6. Construction Fund of Guangxi Health Commission Key Laboratory of Basic Research in Sphingolipid Metabolism Related Diseases (the Affiliated Hospital of Guilin Medical University) [2019B12]
  7. Construction Fund of Key Disciplines of Medical and Health in Guangxi [ZJC2020005]
  8. [2021-8-4-3]

向作者/读者索取更多资源

This study analyzed the proteomic signatures of small extracellular vesicles (sEVs) from plasma samples of hepatocellular carcinoma (HCC) patients. The complement cascade was found to be significantly involved in HCC progression, and four proteins (C1QB, C1QC, C4BPA, and C4BPB) were identified as potential molecular signatures in the plasma sEVs of HCC patients.
Purpose: Liver cancer is one of the most common tumors with the seventh-highest incidence and the third-highest mortality. Many studies have shown that small extracellular vesicles (sEVs) play an important role in liver cancer. Here, we report comprehensive signatures for sEV proteins from plasma obtained from patients with hepatocellular carcinoma (HCC), which might be valuable for the evaluation and diagnosis of HCC. Methods: We extracted sEVs from the plasma of controls and patients with HCC. Differentially expressed proteins in the sEVs were analyzed using label-free quantification and bioinformatic analyses. Western blotting (WB) was used to validate the abovementioned sEV proteins. Results: Proteomic analysis was performed for plasma sEVs from 21 patients with HCC and 15 controls. Among the 335 identified proteins in our study, 27 were significantly dysregulated, including 13 upregulated proteins that were involved predominantly in the complement cascade (complement C1Q subcomponent subunit B (C1QB), complement C1Q subcomponent subunit C (C1QC), C4B-binding protein alpha chain (C4BPA), and C4B-binding protein beta chain (C4BPB)) and the coagulation cascade (F13B, fibrinogen alpha chain (FGA), fibrinogen beta chain (FGB), and fibrinogen gamma chain (FGG)). We verified increased levels of the C1QB, C1QC, C4BPA, and C4BPB proteins in the plasma sEVs from patients with HCC in both the discovery cohort and validation cohort. Conclusions: The complement cascade in sEVs was significantly involved in HCC progression. C1QB, C1QC, C4BPA, and C4BPB were highly abundant in the plasma sEVs from patients with HCC and might represent molecular signatures.

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