期刊
VIROLOGY
卷 577, 期 -, 页码 16-23出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2022.10.005
关键词
Zika virus; Dengue virus; Platelets; MEG-01; NS1
类别
资金
- Ministerio de Ciencia, Tecnologia e Innovacion, Colombia [80740-804-2019, 856]
- Universidad Surcolombiana
This study investigated the effects of DENV-2 and ZIKV infection on human platelet precursors, revealing that DENV-2 induced higher levels of cell death, gene expression, and reduced release of PLPs compared to ZIKV infection.
Platelet count is widely used for the diagnosis and follow-up of patients with dengue. Despite its close viral structural and symptomatic homology, ZIKV infection does not typically induce significant thrombocytopenia. To determine the effect of DENV-2 and ZIKV infection on human platelet precursors we utilized MEG-01 cell line to evaluate the viral infection, viability, innate gene expression and release of platelet-like particles (PLPs). DENV-2 induced a higher proportion of cell death at 48-72 h post-infection than ZIKV. The median range of intracellular NS1+/E+ cells was 11.2% (3.3%-25%) and 5% (3%-8.1%) for DENV-2 and ZIKV, respectively (p = 0.03). MEG-01 cells infected with DENV-2 quickly expressed higher levels of IFN-beta, indolamine 2,3-dioxygenase and CXCL10 mRNA compared to ZIKV infected cells and DENV-2 but not ZIKV infection reduced the number PLPs from stimulated MEG-01 cells. The results shed light into mechanisms including thrombocytopenia present in patients with DENV but absent in ZIKV infections.
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